SUCLG1 Polyclonal antibody 14923-1-AP proteintech
$149.00
In stock
SKU
14923-1-AP
| Catalog Number | 14923-1-AP |
| Synonyms | EC:6.2.1.4, G ALPHA, SCS alpha, SCS-alpha, Succinate--CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrial |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF-P, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HepG2 cells, rat liver tissue, A549 cells |
| Tested Applications — Positive IP detected in | mouse kidney tissue |
| Tested Applications — Positive IHC detected in | mouse kidney tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF-P detected in | mouse brain tissue |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:10000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Recommended Dilutions — Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Positive WB detected in | HepG2 cells, rat liver tissue, A549 cells |
| Positive IP detected in | mouse kidney tissue |
| Positive IHC detected in | mouse kidney tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
| Western Blot (WB) | WB : 1:5000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag6481 Product name: Recombinant human SUCLG1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-333 aa of BC000504 Sequence: MVSGSSGLAAARLLSRSFLLPQNGIRHCSYTASRQHLYVDKNTKIICQGFTGKQGTFHSQQALEYGTKLVGGTTPGKGGQTHLGLPVFNTVKEAKEQTGATASVIYVPPPFAAAAINEAIEAEIPLVVCITEGIPQQDMVRVKHKLLRQEKTRLIGPNCPGVINPGECKIGIMPGHIHKKGRIGIVSRSGTLTYEAVHQTTQVGLGQSLCVGIGGDPFNGTDFIDCLEIFLNDSATEGIILIGEIGGNAEENAAEFLKQHNSGPNSKPVVSFIAGLTAPPGRRMGHAGAIIAGGKGGAKEKISALQSAGVVVSMSPAQLGTTIYKEFEKRKML Predict reactive species |
| Full Name | succinate-CoA ligase, alpha subunit |
| Calculated Molecular Weight | 35 kDa |
| Observed Molecular Weight | 35 kDa |
| GenBank Accession Number | BC000504 |
| Gene Symbol | SUCLG1 |
| Gene ID (NCBI) | 8802 |
| RRID | AB_2197177 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P53597 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for SUCLG1 antibody 14923-1-AP | Download protocol |
| IHC protocol for SUCLG1 antibody 14923-1-AP | Download protocol |
| IP protocol for SUCLG1 antibody 14923-1-AP | Download protocol |
| WB protocol for SUCLG1 antibody 14923-1-AP | Download protocol |
| mouse | WB Cell Death Discov Targeted silencing of CLYBL with platelet-mimetic siRNA nanoparticles drives itaconate-mediated macrophage reprogramming and protects against sepsis-triggered lung cell death. Authors - ZuoJun Huang View Article |
| human | WB bioRxiv Succinate dehydrogenase activity supports de novo purine synthesis Authors - Mushtaq A Nengroo View Article |
| Citations | 18 |
| Dilutions | WB : 1:5000-1:10000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 IF-P : 1:200-1:800 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
SUCLG1(succinyl-CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrial) is also named as SCS-alpha and belongs to the succinate/malate CoA ligase alpha subunit family. The SUCLG1 gene encodes a 35 kDa protein with a 40 amino acids transit peptide. This enzyme catalyzes the ATP- or GTP-dependent ligation of succinate and CoA to form succinyl-CoA. Defects in SUCLG1 are the cause of mitochondrial DNA depletion syndrome type 9 (MTDPS9). Protocols Product Specific Protocols IF protocol for SUCLG1 antibody 14923-1-AP Download protocol IHC protocol for SUCLG1 antibody 14923-1-AP Download protocol IP protocol for SUCLG1 antibody 14923-1-AP Download protocol WB protocol for SUCLG1 antibody 14923-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS.
Journal: Nat Struct Mol Biol | Authors: Xinxin Zuo | Species: mouse | Application: WB - Quantitative subcellular acyl-CoA analysis reveals distinct nuclear metabolism and isoleucine-dependent histone propionylation
Journal: Mol Cell | Authors: Sophie Trefely | Species: human | Application: WB - A genetically encoded fluorescent biosensor for detecting itaconate with subcellular resolution in living macrophages
Journal: Nat Commun | Authors: Pengkai Sun | Species: mouse | Application: WB - Mitochondrial protein hyperacetylation underpins heart failure with preserved ejection fraction in mice.
Journal: J Mol Cell Cardiol | Authors: Xin Liu | Species: mouse | Application: WB,IP - Succinate dehydrogenase activity supports de novo purine synthesis
Journal: bioRxiv | Authors: Mushtaq A Nengroo | Species: human | Application: WB - Targeted silencing of CLYBL with platelet-mimetic siRNA nanoparticles drives itaconate-mediated macrophage reprogramming and protects against sepsis-triggered lung cell death.
Journal: Cell Death Discov | Authors: ZuoJun Huang | Species: mouse | Application: WB - Echinacoside modulates PARP14-GLUD1 axis to mediate energy metabolism reprogramming and mitochondrial function in diminished ovarian reserve.
Journal: Phytomedicine | Authors: Huizhen Liang | Species: rat,human | Application: WB - O-GlcNAcylation Facilitates the Interaction between Keratin 18 and Isocitrate Dehydrogenases and Potentially Influencing Cholangiocarcinoma Progression
Journal: ACS Cent Sci | Authors: Xiangfeng Meng - Biomarker identification and risk prediction model development for differentiated thyroid carcinoma lung metastasis based on primary lesion proteomics
Journal: Clin Cancer Res | Authors: Xiaoqi Peng | Species: human | Application: IHC - Targeted silencing of CLYBL with platelet-mimetic siRNA nanoparticles drives itaconate-mediated macrophage reprogramming and protects against sepsis-triggered lung cell death.
Journal: Cell Death Discov | Authors: ZuoJun Huang | Species: mouse | Application: WB - TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS.
Journal: Nat Struct Mol Biol | Authors: Xinxin Zuo | Species: mouse | Application: WB - Ketogenic β-hydroxybutyrate regulates β-hydroxybutyrylation of TCA cycle-associated enzymes and attenuates disease-associated pathologies in Alzheimer's mice
Journal: Aging Cell | Authors: Wanhong Han | Species: mouse | Application: WB - Acrolein exposure affects ovarian function by interfering with glycolysis and mitochondrial energy metabolism in mouse
Journal: Environ Pollut | Authors: Yan-Zhu Chen | Species: mouse | Application: WB - SIRT5 mediated succinylation of SUCLA2 regulates TCA cycle dysfunction and mitochondrial damage in pancreatic acinar cells in acute pancreatitis
Journal: Biochim Biophys Acta Mol Basis Dis | Authors: Wenbin Liu | Species: rat | Application: WB - Mitochondrial protein hyperacetylation underpins heart failure with preserved ejection fraction in mice.
Journal: J Mol Cell Cardiol | Authors: Xin Liu | Species: mouse | Application: WB,IP - Acrylamide Exposure Impairs Ovarian Tricarboxylic Acid Cycle and Reduces Oocyte Quality in Mouse
Journal: Environ Toxicol | Authors: Yue-Cen Liu | Species: mouse | Application: WB - MLL3/4 methyltransferases regulate the differentiation of pluripotent stem cells through coordinating glycolysis and mitochondrial respiration.
Journal: bioRxiv | Authors: Suzu Mohammad Nur | Species: mouse | Application: WB - Succinate dehydrogenase activity supports de novo purine synthesis
Journal: bioRxiv | Authors: Mushtaq A Nengroo | Species: human | Application: WB - NSUN2 Promotes Cancer Immune Evasion via Its Moonlighting Function Acting on Metabolic Reprogramming.
Journal: Cancer Commun (Lond) | Authors: Baoxiang Chen | Species: human, mouse | Application: WB
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
