SURF2 Polyclonal antibody 16522-1-AP proteintech

$149.00
In stock
SKU
16522-1-AP
Catalog No.SizePrice (USD)
16522-1-AP-20UL20 μL$149.00
16522-1-AP-150UL150 μL$449.00
Catalog Number16522-1-AP
SynonymsSURF 2, Surf-2, surfeit 2
HostRabbit
Reactivityhuman, mouse, rat
FormLiquid
FormulationPBS, Azide, Glycerol
ApplicationsWB, IHC, IF/ICC, ELISA
Host / IsotypeRabbit / IgG
Tested Applications — Positive WB detected inBxPC-3 cells, HeLa cells, COLO 320 cells, HEK-293 cells
Tested Applications — Positive IHC detected inhuman breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Tested Applications — Positive IF/ICC detected inHeLa cells
Recommended Dilutions — Western Blot (WB)WB : 1:500-1:2000
Recommended Dilutions — Immunohistochemistry (IHC)IHC : 1:50-1:500
Recommended Dilutions — Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Positive WB detected inBxPC-3 cells, HeLa cells, COLO 320 cells, HEK-293 cells
Positive IHC detected inhuman breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Tested Reactivityhuman, mouse, rat
ClassPolyclonal
TypeAntibody
ImmunogenCatNo: Ag9745 Product name: Recombinant human SURF2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-256 aa of BC014411 Sequence: MSELPGDVRAFLREHPSLRLQTDARKVRCILTGHELPCRLPELQVYTRGKKYQRLVRASPAFDYAEFEPHIVPSTKNPHQLFCKLTLRHINKCPEHVLRHTQGRRYQRALCKYEECQKQGVEYVPACLVHRRRRREDQMDGDGPRPREAFWEPTSSDEGGAASDDSMTDLYPPELFTRKDLGSTEDGDGTDDFLTDKEDEKAKPPREKATDEGRRETTVYRGLVQKRGKKQLGSLKKKFKSHHRKPKSFSSCKQPG Predict reactive species
Full Namesurfeit 2
Calculated Molecular Weight256 aa, 30 kDa
Observed Molecular Weight35-40 kDa
GenBank Accession NumberBC014411
Gene SymbolSURF2
Gene ID (NCBI)6835
RRIDAB_2255415
ConjugateUnconjugated
Purification MethodAntigen affinity purification
UNIPROT IDQ15527
Storage BufferPBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.
IF protocol for SURF2 antibody 16522-1-APDownload protocol
IHC protocol for SURF2 antibody 16522-1-APDownload protocol
WB protocol for SURF2 antibody 16522-1-APDownload protocol
Citations-
DilutionsWB : 1:500-1:2000 IHC : 1:50-1:500 IF/ICC : 1:50-1:500
IsotypeIgG
ClonalityPolyclonal
Clone Number-
ConjugationUnconjugated

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