TAGLN3 Polyclonal antibody 12246-1-AP proteintech
$149.00
In stock
SKU
12246-1-AP
| Catalog Number | 12246-1-AP |
| Synonyms | transgelin 3, Neuronal protein 22, Neuronal protein NP25, NP25, Transgelin-3 |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | fetal human brain tissue, mouse brain tissue, rat brain tissue |
| Tested Applications — Positive IP detected in | rat brain tissue, mouse brain tissue |
| Tested Applications — Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Positive WB detected in | fetal human brain tissue, mouse brain tissue, rat brain tissue |
| Positive IP detected in | rat brain tissue, mouse brain tissue |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag2891 Product name: Recombinant human TAGLN3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-199 aa of BC015329 Sequence: MANRGPSYGLSREVQEKIEQKYDADLENKLVDWIILQCAEDIEHPPPGRAHFQKWLMDGTVLCKLINSLYPPGQEPIPKISESKMAFKQMEQISQFLKAAETYGVRTTDIFQTVDLWEGKDMAAVQRTLMALGSVAVTKDDGCYRGEPSWFHRKAQQNRRGFSEEQLRQGQNVIGLQMGSNKGASQAGMTGYGMPRQIM Predict reactive species |
| Full Name | transgelin 3 |
| Calculated Molecular Weight | 22.4 kDa |
| Observed Molecular Weight | 22.4 kDa |
| GenBank Accession Number | BC015329 |
| Gene Symbol | TAGLN3 |
| Gene ID (NCBI) | 29114 |
| RRID | AB_2199509 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UI15 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for TAGLN3 antibody 12246-1-AP | Download protocol |
| IP protocol for TAGLN3 antibody 12246-1-AP | Download protocol |
| WB protocol for TAGLN3 antibody 12246-1-AP | Download protocol |
| mouse | IHC Sci Rep microRNA regulatory circuits in a mouse model of inherited retinal degeneration. Authors - Arpad Palfi View Article |
| Citations | 1 |
| Dilutions | WB : 1:5000-1:50000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:20-1:200 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
The transgelin family is a group of proteins that belong to 22 kDa actin-related corpnin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22 alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells. This antibody was generated against full length transgenlin 3 protein. It may cross-react with other two transgenlins based on the sequence similarity. Protocols Product Specific Protocols IHC protocol for TAGLN3 antibody 12246-1-AP Download protocol IP protocol for TAGLN3 antibody 12246-1-AP Download protocol WB protocol for TAGLN3 antibody 12246-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- microRNA regulatory circuits in a mouse model of inherited retinal degeneration.
Journal: Sci Rep | Authors: Arpad Palfi | Species: mouse | Application: IHC
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