TIMELESS Monoclonal antibody 67022-1-Ig proteintech
$149.00
In stock
SKU
67022-1-Ig
| Catalog Number | 67022-1-Ig |
| Synonyms | hTIM, Protein timeless homolog, TIM, TIM1, timeless homolog (Drosophila) |
| Host | Mouse |
| Reactivity | human, mouse, rat and More (1) |
| Reactivity | human, mouse, rat, canine |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, FC (Intra), ELISA |
| Host / Isotype | Mouse / IgG1 |
| Tested Applications — Positive WB detected in | HeLa cells, U2OS cells, A549 cells, JAR cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, HEK-293 cells, K-562 cells |
| Tested Applications — Positive FC (Intra) detected in | HEK-293 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Recommended Dilutions — Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Positive WB detected in | HeLa cells, U2OS cells, A549 cells, JAR cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, HEK-293 cells, K-562 cells |
| Positive FC (Intra) detected in | HEK-293 cells |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, canine |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag6283 Product name: Recombinant human TIMELESS protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 869-1208 aa of BC050557 Sequence: QMGLADSVKDFQRKGTHIVLWTGDQELELQRLFEEFRDSDDVLGHIMKNITAKRSRARIVDKLLALGLVAERRELYKKRQKKLASSILPNGAESLKDFCQEDLEEEENLPEEDSEEEEEGGSEAEQVQGSLVLSNENLGQSLHQEGFSILLLWLQNCLIRAADDREEDGCSQAVPLVPLTEENEEAMENEQFQQLLRKLGVRPPASGQETFWRIPAKLSPTQLRRAAASLSQPEEEQKLQPELQPKVPGEQGSDEEHCKEHRAQALRALLLAHKKKAGLASPEEEDAVGKEPLKAAPKKRQLLDSDEEQEEDEGRNRAPELGAPGIQKKKRYQIEDDEDD Predict reactive species |
| Full Name | timeless homolog (Drosophila) |
| Calculated Molecular Weight | 139 kDa |
| Observed Molecular Weight | 140 kDa |
| GenBank Accession Number | BC050557 |
| Gene Symbol | TIMELESS |
| Gene ID (NCBI) | 8914 |
| RRID | AB_2882337 |
| Conjugate | Unconjugated |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9UNS1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| FC protocol for TIMELESS antibody 67022-1-Ig | Download protocol |
| WB protocol for TIMELESS antibody 67022-1-Ig | Download protocol |
| human,canine | WB Eur J Pharmacol TIMELESS promotes the proliferation and migration of lung adenocarcinoma cells by activating EGFR through AMPK and SPHK1 regulation Authors - Houqing Yin KD Validated View Article |
| Citations | 2 |
| Dilutions | WB : 1:5000-1:50000 FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Clone Number | 1B6G3 |
| Conjugation | Unconjugated |
TIMELESS1 (TIM1) gene encodes protein timeless homolog belonging to timeless family. TIM1 is localized in nucleus and regulated by cell cycle. It expresses high levels in S, G2 and M phases, and low in G0 and G1 phases. TIM1 is involved in the circadian rhythm autoregulatory loop and is a crucial component of the circadian core oscillator, which includes the CRY proteins, CLOCK, NPAS2, etc. It is also involved in cell survival after DNA damage or replication stress and reported to play important role in epithelial cell morphogenesis and formation of branching tubules. Protocols Product Specific Protocols FC protocol for TIMELESS antibody 67022-1-Ig Download protocol WB protocol for TIMELESS antibody 67022-1-Ig Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- TIMELESS promotes the proliferation and migration of lung adenocarcinoma cells by activating EGFR through AMPK and SPHK1 regulation
Journal: Eur J Pharmacol | Authors: Houqing Yin KD Validated | Species: human,canine | Application: WB - TIMELESS promotes glioma stemness and malignancy through JAK-STAT3 pathway.
Journal: J Biol Chem | Authors: Yuichiro Kai
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