TTF2 Polyclonal antibody 13722-1-AP proteintech
$149.00
In stock
SKU
13722-1-AP
| Catalog Number | 13722-1-AP |
| Synonyms | EC:3.6.4.-, F2, HuF2 |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | A2780 cells, HeLa cells, Jurkat cells |
| Tested Applications — Positive IP detected in | A2780 cells |
| Tested Applications — Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HeLa cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:2000-1:10000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Positive WB detected in | A2780 cells, HeLa cells, Jurkat cells |
| Positive IP detected in | A2780 cells |
| Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag4671 Product name: Recombinant human TTF2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-300 aa of BC030058 Sequence: MEEVRCPEHGTFCFLKTGVRDGPNKGKSFYVCRADTCSFVRATDIPVSHCLLHEDFVVELQGLLLPQDKKEYRLFFRCIRSKAEGKRWCGSIPWQDPDSKEHSVSNKSQHASETFHHSSNWLRNPFKVLDKNQEPALWKQLIKGEGEEKKADKKQREKGDQLFDQKKEQKPEMMEKDLSSGLVPKKKQSVVQEKKQEEGAEIQCEAETGGTHKRDFSEIKSQQCQGNELTRPSASSQEKSSGKSQDVQRESEPLREKVTQLLPQNVHSHNSISKPQKGGPLNKEYTNWEAKETKAKDGPS Predict reactive species |
| Full Name | transcription termination factor, RNA polymerase II |
| Calculated Molecular Weight | 1162 aa, 129 kDa |
| Observed Molecular Weight | 130 kDa |
| GenBank Accession Number | BC030058 |
| Gene Symbol | TTF2 |
| Gene ID (NCBI) | 8458 |
| RRID | AB_2262865 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UNY4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for TTF2 antibody 13722-1-AP | Download protocol |
| IHC protocol for TTF2 antibody 13722-1-AP | Download protocol |
| IP protocol for TTF2 antibody 13722-1-AP | Download protocol |
| WB protocol for TTF2 antibody 13722-1-AP | Download protocol |
| human | IHC Nat Commun Single-cell analysis of gastric signet ring cell carcinoma reveals cytological and immune microenvironment features Authors - Weizhu Zhao View Article |
| rat | WB Sci Total Environ PKCα mediated by the PI3K/Akt-FOXA1 cascade facilitates cypermethrin-induced hyperthyroidism. Authors - Mei Ha View Article |
| Citations | 3 |
| Dilutions | WB : 1:2000-1:10000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:20-1:200 IF/ICC : 1:200-1:800 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Transcription elongation is an key target for control of eukaryotic gene expression. After initiating from a promoter, the RNA polymerase II elongation complex is subjected to a block to elongation by the action of components of N-TEF (negative transcription elongation factor) [PMID: 9748214]. Transcription termination factor 2 (TTF2) is a DsDNA-dependent ATPase that acts as a transcription termination factor by coupling ATP hydrolysis with removal of RNA polymerase II from the DNA template. It also contribute to mitotic transcription repression, and involved in pre-mRNA splicing[PMID:15125840, 12927788]. Protocols Product Specific Protocols IF protocol for TTF2 antibody 13722-1-AP Download protocol IHC protocol for TTF2 antibody 13722-1-AP Download protocol IP protocol for TTF2 antibody 13722-1-AP Download protocol WB protocol for TTF2 antibody 13722-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- mRNA decapping factors and the exonuclease Xrn2 function in widespread premature termination of RNA polymerase II transcription.
Journal: Mol Cell | Authors: Brannan Kris K | Species: human | Application: WB - PKCα mediated by the PI3K/Akt-FOXA1 cascade facilitates cypermethrin-induced hyperthyroidism.
Journal: Sci Total Environ | Authors: Mei Ha | Species: rat | Application: WB - Single-cell analysis of gastric signet ring cell carcinoma reveals cytological and immune microenvironment features
Journal: Nat Commun | Authors: Weizhu Zhao | Species: human | Application: IHC
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