TUB Polyclonal antibody 17928-1-AP proteintech
$149.00
In stock
SKU
17928-1-AP
| Catalog Number | 17928-1-AP |
| Synonyms | rd5 |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, IP, ChIP, ELISA |
| Applications | WB, IHC, IF/ICC, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | mouse brain tissue, mouse uterus tissue, Neuro-2a cells, rat brain tissue |
| Tested Applications — Positive IP detected in | mouse brain tissue |
| Tested Applications — Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | Neuro-2a cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:2000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Positive WB detected in | mouse brain tissue, mouse uterus tissue, Neuro-2a cells, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Neuro-2a cells |
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag12354 Product name: Recombinant human TUB protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 212-561 aa of BC075032 Sequence: GQSDHAQDAGETAAGGGERPSGQDLRATMQRKGISSSMSFDEDEEDEEENSSSSSQLNSNTRPSSATSRKSVREAASAPSPTAPEQPVDVEVQDLEEFALRPAPQGITIKCRITRDKKGMDRGMYPTYFLHLDREDGKKVFLLAGRKRKKSKTSNYLISVDPTDLSRGGDSYIGKLRSNLMGTKFTVYDNGVNPQKASSSTLESGTLRQELAAVCYETNVLGFKGPRKMSVIVPGMNMVHERVSIRPRNEHETLLARWQNKNTESIIELQNKTPVWNDDTQSYVLNFHGRVTQASVKNFQIIHGNDPDYIVMQFGRVAEDVFTMDYNYPLCALQAFAIALSSFDSKLACE Predict reactive species |
| Full Name | tubby homolog (mouse) |
| Calculated Molecular Weight | 561 aa, 62 kDa |
| Observed Molecular Weight | 56 kDa, 62-67 kDa |
| GenBank Accession Number | BC075032 |
| Gene Symbol | TUB |
| Gene ID (NCBI) | 7275 |
| RRID | AB_2212236 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P50607 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for TUB antibody 17928-1-AP | Download protocol |
| IHC protocol for TUB antibody 17928-1-AP | Download protocol |
| IP protocol for TUB antibody 17928-1-AP | Download protocol |
| WB protocol for TUB antibody 17928-1-AP | Download protocol |
| human | WB,ChIP Cell Rep NAD+ supplement potentiates tumor-killing function by rescuing defective TUB-mediated NAMPT transcription in tumor-infiltrated T cells. Authors - Yuetong Wang KD Validated View Article |
| mouse | WB,IF Cell Commun Signal miR-10b-5p regulates adipocyte lineage commitment and adipogenesis via targeting of Gata6 and Tubby. Authors - Nikoletta Kalenderoglou View Article |
| Citations | 6 |
| Dilutions | WB : 1:500-1:2000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:20-1:200 IF/ICC : 1:200-1:800 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Publications
- Differential Roles of Tubby Family Proteins in Ciliary Formation and Trafficking.
Journal: Mol Cells | Authors: Julie J Hong | Species: human | Application: WB - NAD+ supplement potentiates tumor-killing function by rescuing defective TUB-mediated NAMPT transcription in tumor-infiltrated T cells.
Journal: Cell Rep | Authors: Yuetong Wang KD Validated | Species: human | Application: WB,ChIP - Distribution of ciliary adaptor proteins tubby and TULP3 in the organ of Corti
Journal: Front Neurosci | Authors: Laura A Lindner | Species: mouse | Application: IF - The TUB variant impairs leptin sensitivity and AgRP neuronal response, leading to obesity
Journal: Sci Transl Med | Authors: Muye Tong - TMEM145 is a principal component of outer hair cell stereocilia.
Journal: Neuron | Authors: Dennis Derstroff | Species: mouse | Application: IHC - miR-10b-5p regulates adipocyte lineage commitment and adipogenesis via targeting of Gata6 and Tubby.
Journal: Cell Commun Signal | Authors: Nikoletta Kalenderoglou | Species: mouse | Application: WB,IF
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