ZNF638 Polyclonal antibody 31300-1-AP proteintech
$149.00
In stock
SKU
31300-1-AP
| Catalog Number | 31300-1-AP |
| Synonyms | CTCL associated antigen se33 1, NP220, Nuclear protein 220, ZFML, zinc finger protein 638 |
| Host | Rabbit |
| Reactivity | human |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HEK-293T cells, HeLa cells, HepG2 cells, Jurkat cells |
| Tested Applications — Positive IP detected in | Jurkat cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:1000-1:8000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Positive WB detected in | HEK-293T cells, HeLa cells, HepG2 cells, Jurkat cells |
| Positive IP detected in | Jurkat cells |
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Tested Reactivity | human |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag35296 Product name: Recombinant human ZNF638 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1777-1978 aa of NM_014497.4 Sequence: VDEVGEEEDGDNDLKVELAQSKNDHPTDKKGNRKKRAVDTKKTKLESLSQVGPVNENVMEEDLKTMIERHLTAKTPTKRVRIGKTLPSEKAVVTEPAKGEEAFQMSEVDEESGLKDSEPERKRKKTEDSSSGKSVASDVPEELDFLVPKAGFFCPICSLFYSGEKAMTNHCKSTRHKQNTEKFMAKQRKEKEQNEAEERSSR Predict reactive species |
| Full Name | zinc finger protein 638 |
| Calculated Molecular Weight | 221kDa |
| Observed Molecular Weight | 270-300 kDa |
| GenBank Accession Number | NM_014497.4 |
| Gene Symbol | ZNF638 |
| Gene ID (NCBI) | 27332 |
| RRID | AB_3669937 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q14966 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IP protocol for ZNF638 antibody 31300-1-AP | Download protocol |
| WB protocol for ZNF638 antibody 31300-1-AP | Download protocol |
| Moritz (Verified Customer) (09-26-2025) | Several band are visible in the area between 150-250 kDa Applications: Western Blot Primary Antibody Dilution: 1:2000 Cell Tissue Type: HEK293T ZNF638 Antibody Western Blot validation (1:2000 dilution) in HEK293T (Cat no:31300-1-AP) |
| Aleksandr (Verified Customer) (07-29-2025) | Antibody recognise a band of the expected size with some other prominent, likely non-specific ones Applications: Western Blot Primary Antibody Dilution: 1:2000 Cell Tissue Type: RKO ZNF638 Antibody Western Blot validation (1:2000 dilution) in RKO (Cat no:31300-1-AP) |
| Tsimafei (Verified Customer) (08-07-2024) | Great antibody Applications: Western Blot Primary Antibody Dilution: 1:3000 Cell Tissue Type: U2OS ZNF638 Antibody Western Blot validation (1:3000 dilution) in U2OS (Cat no:31300-1-AP) |
| Julia (Verified Customer) (08-07-2024) | Band below 250 kDa marker is likely the Znf639 (~221kDa), there is also strong signal between 100-50kDa. I used 30ug of mESC protein extract. Applications: Western Blot Primary Antibody Dilution: 1:5000 Cell Tissue Type: mESC ZNF638 Antibody Western Blot validation (1:5000 dilution) in mESC (Cat no:31300-1-AP) |
| Citations | 1 |
| Dilutions | WB : 1:1000-1:8000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
ZNF638 (Zinc finger protein 638) is also named as NP220 and ZFML. ZNF638 is a newly discovered member of zinc finger protein family for adipocyte function in vitro. And ZNF638regulates triglyceride metabolism via ANGPTL8 in an estrogen dependent manner (PMID: 38211696). ZNF638 plays a role as a transcriptional coregulator of adipocyte differentiation via induction of PPARγ in cooperation with CCAAT/enhancer binding proteins (C/EBPs) (PMID: 25024404). ZNF638 is localized in nuclear bodies enriched with splicing factors (PMID: 25024404). The ZNF638 protein is modified and exhibits a molecular weight of ~300 kDa (PMID: 33040080) Protocols Product Specific Protocols IP protocol for ZNF638 antibody 31300-1-AP Download protocol WB protocol for ZNF638 antibody 31300-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- An integrative epigenomic and single-cell framework identifies a prognostic DNA methylation risk score and ZNF638 silencing in urothelial carcinoma.
Journal: J Transl Med | Authors: Bin Guo
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