CoraLite®594-conjugated Alpha smooth muscle actin specific Monoclonal antibody CL594-67735 proteintech
$479.00
In stock
SKU
CL594-67735
| Catalog Number | CL594-67735 |
| Synonyms | ACTA2, Alpha SMA, a-SMA, smooth muscle actin, 1E9A11 |
| Host | Mouse |
| Reactivity | human, mouse, rat, pig |
| Form | Liquid |
| Formulation | PBS, Proclin300, BSA, Glycerol |
| Applications | IF-P, FC (Intra) |
| Host / Isotype | Mouse / IgG1 |
| Tested Applications — Positive IF-P detected in | mouse heart tissue |
| Tested Applications — Positive FC (Intra) detected in | C2C12 cells |
| Recommended Dilutions — Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Recommended Dilutions — Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Positive IF-P detected in | mouse heart tissue |
| Positive FC (Intra) detected in | C2C12 cells |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | rat |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Peptide Predict reactive species |
| Full Name | actin, alpha 2, smooth muscle, aorta |
| Calculated Molecular Weight | 42 kDa |
| Observed Molecular Weight | 42 kDa |
| GenBank Accession Number | NM_001613 |
| Gene Symbol | Alpha smooth muscle actin |
| Gene ID (NCBI) | 59 |
| RRID | AB_2920177 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
| Excitation Laser | Yellow-Green Laser (561 nm) |
| Purification Method | Protein G purification |
| UNIPROT ID | P62736 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. |
| FC protocol for CL594 Alpha smooth muscle actin specific antibody CL594-67735 | Download protocol |
| IF protocol for CL594 Alpha smooth muscle actin specific antibody CL594-67735 | Download protocol |
| rat | IF Acta Biomater Polyethyleneimine-modified eggshell membrane for dual bone and periosteum regeneration: A multifunctional approach for guided bone healing Authors - Miaozhu Lin View Article |
| Citations | 4 |
| Dilutions | IF-P : 1:50-1:500 FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Clone Number | 1E9A11 |
| Conjugation | CoraLite®594 |
ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells. Protocols Product Specific Protocols FC protocol for CL594 Alpha smooth muscle actin specific antibody CL594-67735 Download protocol IF protocol for CL594 Alpha smooth muscle actin specific antibody CL594-67735 Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- A functional cardiac patch promotes cardiac repair by modulating the CCR2- cardiac-resident macrophage niche and their cell crosstalk
Journal: Cell Rep Med | Authors: Chengbin Ding | Application: IF - Polyethyleneimine-modified eggshell membrane for dual bone and periosteum regeneration: A multifunctional approach for guided bone healing
Journal: Acta Biomater | Authors: Miaozhu Lin | Species: rat | Application: IF - MFAP4 Promotes Scar Formation by Regulating Macrophage-Myofibroblast Transformation Through Activation of the TGF-β/Smad3 Signalling Pathway.
Journal: Exp Dermatol | Authors: Zhu Zhu - A 3D-printed extravascular stent containing sirtuin-3 engineered human bone marrow mesenchymal stem cells maintains venous graft patency.
Journal: Stem Cell Res Ther | Authors: Kexun Liu
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