Mouse CD68 ELISA Kit KE10112 proteintech
$299.00
In stock
SKU
KE10112
| Product name | Mouse CD68 ELISA Kit |
| Synonyms | Cd68, CD68 antigen, gp110, Scard1 |
| Reactivity | Mouse |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Cell culture supernatant，Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.7 pg/mL |
| Range | 7.8-500 pg/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 12514 |
| Sample Type | Average Range |
| Cell culture supernatant | 89% 78%-101% |
| Cell lysate | 99% 92%-113% |
| Sample | n mean ( pg/mL ) SD CV% |
| 1 | 24 266.1 21.9 8.2 |
| 2 | 24 61.9 5.2 8.4 |
| 3 | 24 13.7 0.7 5.3 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
CD68 is a 110 kDa transmembrane glycoprotein widely expressed in monocyte cell types, such as macrophages, microglia, and osteoclasts. It is located at the lysosome and plasma membrane and is essential in various physiological and pathological processes, including atherosclerosis formation, inflammation and auto-immunity, bone-resorbing promotion, and tumor progression. It participates in the cellular response to lipopolysaccharide/oxidized low-density lipoprotein particle stimulus and negative regulation of dendritic cell antigen processing and presentation. Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms Cd68, CD68 antigen, gp110, Scard1 FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
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