Speedy™ Mouse TNFRSF9/CD137 One-Step ELISA Kit SE60051 proteintech
$449.00
In stock
SKU
SE60051
| Product name | Speedy™ Mouse TNFRSF9/CD137 One-Step ELISA Kit |
| Synonyms | 4-1BB, 4-1BB ligand receptor, CD137, Ila, Ly63 |
| Reactivity | Mouse |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 2.7 pg/mL |
| Range | 15.6-1000 pg/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 21942 |
| Sample Type | Average Range |
| Mouse serum | 108% 105%-112% |
| Cell lysate | 101% 99%-102% |
| Sample | n mean ( pg/mL ) SD CV% |
| 1 | 16 501.8 41.0 8.2 |
| 2 | 16 129.8 9.7 7.5 |
| 3 | 16 65.2 5.8 8.9 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
Tumor necrosis factor receptor superfamily member 9 (TNFRSF9, also known as CD137 and 4-1BB) is a member of the TNF receptor superfamily. It is an inducible T cell surface receptor that plays a crucial role in the development and activation of multiple immune cells. The interaction of CD137 with its ligand, 4-1BBL, costimulates T cell proliferation, activation, and survival, and can enhance activation-induced cell death of repetitively stimulated T cells. This receptor also contributes to the development of CD8 cytotoxic T cells and anti-tumor immunity. Moreover, soluble CD137 (sCD137) protein levels were elevated in adipose tissues of obese subjects, and sCD137 enhanced obesity-induced adipose inflammation by triggering CD137L reverse inflammatory signals in macrophages and activated T cells. Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms 4-1BB, 4-1BB ligand receptor, CD137, Ila, Ly63 FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent. 2、If thermostatic shaker is not available, will it affect the duration of my experiment and the experimental data? Answer: If thermostatic shaker is not available, it may take 2.5 hours to complete this experiment. After adding the antibody cocktail, we suggest you incubate the plate at 37℃ for 2 hours. After adding TMB, you only need to incubate the plate at 37℃ for 15 minutes. Through comparative experiments, our R&D personnel have confirmed that you can still obtain valid experimental data, although the signal will be slightly reduced.
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