Speedy™ Rat CD126/IL-6R alpha One-Step ELISA Kit SE70012 proteintech
$449.00
In stock
SKU
SE70012
| Product name | Speedy™ Rat CD126/IL-6R alpha One-Step ELISA Kit |
| Synonyms | CD126, IL-6R alpha, IL-6R 1, Il6r, IL-6RA |
| Reactivity | Rat |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 0.02 ng/mL |
| Range | 0.156-10 ng/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 24499 |
| Sample Type | Average Range |
| Rat serum | 94% 93%-97% |
| Tissue lysate | 93% 83%-100% |
| Sample | n mean ( ng/mL ) SD CV% |
| 1 | 16 4.99 0.11 2.25 |
| 2 | 16 1.16 0.04 3.45 |
| 3 | 16 0.63 0.01 1.59 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
CD126, also known as IL-6R alpha, is a transmembrane protein that forms part of the IL-6 receptor complex. This receptor complex is essential for mediating the biological effects of IL-6, a cytokine involved in various physiological processes, including immune responses, as well as cell growth and differentiation. IL-6R alpha interacts with glycoprotein 130 (gp130) to facilitate the anti-inflammatory and regenerative activities of IL-6. Additionally, the combination of soluble CD126 and gp130 in serum forms a buffer system that neutralizes the excess levels of blood IL-6 in healthy individuals. Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms CD126, IL-6R alpha, IL-6R 1, Il6r, IL-6RA FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent. 2、If thermostatic shaker is not available, will it affect the duration of my experiment and the experimental data? Answer: If thermostatic shaker is not available, it may take 2.5 hours to complete this experiment. After adding the antibody cocktail, we suggest you incubate the plate at 37℃ for 2 hours. After adding TMB, you only need to incubate the plate at 37℃ for 15 minutes. Through comparative experiments, our R&D personnel have confirmed that you can still obtain valid experimental data, although the signal will be slightly reduced.
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