| Product name | Human CXCL8/IL-8 ELISA Kit |
| Synonyms | CXCL8,IL8,IL-8,C X C motif chemokine 8,Chemokine |
| Reactivity | Human |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 0.6 pg/mL |
| Range | 1.56-100 pg/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3576 |
| Sample Type | Average Range |
| Human serum | 98% 93%-107% |
| Cell culture supernatant | 105% 100%-109% |
| Sample | n mean ( pg/mL ) SD CV% |
| 1 | 16 45.8 3.2 7.0 |
| 2 | 16 11.3 1.0 8.8 |
| 3 | 16 5.8 0.5 8.9 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| human | serum,cell culture supernatants Cell Commun Signal Activation of mechanosensitive ion channel Piezo1 linking metabolic reprogramming and pro-inflammatory responses in hepatocellular carcinoma Authors - Juanfen Mo View Article |
| FH Maherah (Verified Customer) (12-12-2025) | Human CXCL8/IL-8 ELISA kit Applications: Other Cell Tissue Type: Human Corneal Epithelial Cells |
Interleukin 8 (IL-8), also known as CXCL8, which is a member of the CXC chemokine family. This chemokine is secreted by a variety of cell types including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, and various tumor cell lines in response to inflammatory stimuli. IL-8 has two primary functions. It induces chemotaxis in target cells, primarily neutrophils but also other granulocytes, causing them to migrate toward the site of infection. IL-8 also induces phagocytosis once they have arrived. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. IL-8 is also known to be a potent promoter of angiogenesis. IL-8 has been associated with tumor angiogenesis, metastasis, and poor prognosis in breast cancer. IL-8 may present a novel therapeutic target for estrogen driven breast carcinogenesis and tumor progression. Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms CXCL8,IL8,IL-8,C X C motif chemokine 8,Chemokine FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
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