Anti-KMT3A/HYPB/HIF-1 Rabbit pAb servicebio GB113441-50
$50.00
In stock
SKU
GB113441-50
Product Information
| Protein full name | Histone-lysine N-methyltransferase SETD2 |
| Synonyms | HIF-1, HIF1, HIP-1, hSET2, HSPC069, Huntingtin yeast partner B, HYPB, KIAA1732, KMT3A, Lysine N methyltransferase 3A, p231HBP, SET domain containing 2, SET2, SETD2 |
| Uniprot ID | E9Q5F9 |
| Immunogen | Recombinant protein corresponding to Mouse KMT3A / HYPB / HIF-1 |
| Isotype | IgG |
| Predicted MW./Observed MW. | 286 kDa / 286 kDa |
| Purity | Affinity purification |
| Subcellular location | Chromosome, Nucleus |
Applications
| Applications: WB | Species: Mouse |
| Dilution: 1: 300-1: 500 | Positive Tissue: brain |
| Applications: IHC/IF | Species: Mouse |
| Dilution: 1: 1800-1: 3600 | Positive Tissue: brain, cerebellum |
Background
SETD2, also named as HIF1, HYPB, KIAA1732, KMT3A, SET2, p231HBP, hSET2 and HIP-1, belongs to the histone-lysine methyltransferase family and SET2 subfamily. SETD2 probably plays a role in chromatin structure modulation during elongation via its interaction with hyperphosphorylated POLR2A. It binds DNA at promoters. SETD2 may also act as a transcription activator that binds to promoters. It is a potential tumour suppressor gene in breast cancer. This antibody is specific to SETD2.
Images
WB analysis of KMT3A/HYPB/HIF-1(GB113441). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.
IHC analysis of KMT3A/HYPB/HIF-1(GB113441). Sample: Mouse cerebellum, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Primary antibody: 1: 1800, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.
IHC analysis of KMT3A/HYPB/HIF-1(GB113441). Sample: Mouse brain, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Primary antibody: 1: 1800, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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