Anti-SerpinB2/PAI-2 Rabbit pAb servicebio GB112219-50
$50.00
In stock
SKU
GB112219-50
Product Information
| Protein full name | Plasminogen activator inhibitor 2, macrophage |
| Synonyms | PAI-2, Serpin B2, Serpinb2, Pai2, Planh2, Monocyte Arginine-serpin, Urokinase inhibitor |
| Uniprot ID | P12388 |
| Immunogen | Recombinant protein corresponding to Mouse SerpinB2/PAI-2 |
| Isotype | IgG |
| Predicted MW./Observed MW. | 46 kDa / 42 kDa |
| Purity | Affinity purification |
| Subcellular location | Cytoplasm, Secreted |
Applications
| Applications: WB | Species: Human, Mouse |
| Dilution: 1: 500-1: 1000 | Positive Tissue: skin |
| Applications: IHC/IF | Species: Human, Mouse, Rat |
| Dilution: 1: 600-1: 1200 | Positive Tissue: Hodgkin's lymphoma, skin |
Background
SerpinB2 is a serine proteinase inhibitor of the ovalbumin like B clade of serpins. It was first discovered in the placenta, and given the name PAI-2 because of the ability to inhibit urokinase plasminogen activator (u-PA) at low micromolar efficiency. SerpinB2 also inhibits tissue plasminogen activator (t-PA), but with micromolar efficiency, and PAI-1 is much more efficient that SerpinB2 on both forms of plasminogen activator. The structure of PAI-2 is not terribly similar to PAI-1, however, which is an E clade serpin.
Images
WB analysis of SerpinB2/PAI-2(GB112219). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.
WB analysis of SerpinB2/PAI-2(GB112219). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.
IF analysis of SerpinB2/PAI-2(GB112219)red. Sample: Mouse skin, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 600, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.
IF analysis of SerpinB2/PAI-2(GB112219)red. Sample: Mouse skin, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 600, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.
IF analysis of SerpinB2/PAI-2(GB112219)red. Sample: Rat skin, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 600, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.
IF analysis of SerpinB2/PAI-2(GB112219)red. Sample: Human Hodgkin's lymphoma, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 600, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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