Anti-RPL10A Rabbit pAb servicebio GB115036-50
$50.00
In stock
SKU
GB115036-50
Product Information
| Protein full name | 60S ribosomal protein L10a |
| Synonyms | 60S ribosomal protein L10a, Csa 19, NEDD 6, NEDD6, ribosomal protein L10a |
| Uniprot ID | P53026 |
| Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse RPL10A |
| Isotype | IgG |
| Predicted MW./Observed MW. | 25 kDa / 30 kDa |
| Purity | Affinity purification |
| Subcellular location | Cytoplasm |
Applications
| Applications: WB | Species: Human, Mouse, Rat |
| Dilution: 1: 1000-1: 2000 | Positive Tissue: heart, liver, lung |
| Applications: IHC/IF | Species: Human, Mouse, Rat |
| Dilution: 1: 500-1: 2000 | Positive Tissue: gastric cancer, kidney, brain |
| Applications: ICC/IF | Species: Mouse |
| Dilution: 1: 100-1: 600 | Positive Tissue: NIH-3T3 |
Background
Component of the large ribosomal subunit.
Images
WB analysis of RPL10A(GB115036). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.
WB analysis of RPL10A(GB115036). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.
WB analysis of RPL10A(GB115036). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.
IHC analysis of RPL10A(GB115036). Sample: Human kidney cancer, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Primary antibody: 1: 600, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.
IHC analysis of RPL10A(GB115036). Sample: Mouse brain, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Primary antibody: 1: 600, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.
IHC analysis of RPL10A(GB115036). Sample: Mouse kidney, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Primary antibody: 1: 600, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.
IHC analysis of RPL10A(GB115036). Sample: Rat brain, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Primary antibody: 1: 600, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.
ICC analysis of RPL10A(GB115036)red. Sample: NIH-3T3, 4% PFA (G1101) 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 400, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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