Anti-LEPREL1 Rabbit pAb servicebio GB115118-100

$50.00
In stock
SKU
GB115118-100

 

Product Information

Protein full nameProlyl 3-hydroxylase 2
Synonymsleprecan like 1, Leprecan like protein 1, LEPREL1, MLAT4, P3H2, Prolyl 3 hydroxylase 2
Uniprot IDQ8CG71
ImmunogenKLH conjugated Synthetic peptide corresponding to Mouse P3H2
IsotypeIgG
Predicted MW./Observed MW.80 kDa / 90 kDa
PurityAffinity purification

Applications

Applications: WBSpecies: Mouse
Dilution: 1: 1500-1: 2000Positive Tissue: kidney

Background

Prolyl 3-hydroxylase that catalyzes the post-translational formation of 3-hydroxyproline on collagens . Contributes to proline 3-hydroxylation of collagen COL4A1 and COL1A1 in tendons, the eye sclera and in the eye lens capsule . Has high activity with the type IV collagen COL4A1, and lower activity with COL1A1. Catalyzes hydroxylation of the first Pro in Gly-Pro-Hyp sequences where Hyp is 4-hydroxyproline. Has no activity on substrates that have proline instead of 4-hydroxyproline in the third position (By similarity).

Images

WB analysis of LEPREL1(GB115118). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1500, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

Storage

StorageStore at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage BufferPBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).

 

 

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