Product Information
| Protein full name | Prolyl 3-hydroxylase 2 |
| Synonyms | leprecan like 1, Leprecan like protein 1, LEPREL1, MLAT4, P3H2, Prolyl 3 hydroxylase 2 |
| Uniprot ID | Q8CG71 |
| Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse P3H2 |
| Isotype | IgG |
| Predicted MW./Observed MW. | 80 kDa / 90 kDa |
| Purity | Affinity purification |
Applications
| Applications: WB | Species: Mouse |
| Dilution: 1: 1500-1: 2000 | Positive Tissue: kidney |
Background
Prolyl 3-hydroxylase that catalyzes the post-translational formation of 3-hydroxyproline on collagens . Contributes to proline 3-hydroxylation of collagen COL4A1 and COL1A1 in tendons, the eye sclera and in the eye lens capsule . Has high activity with the type IV collagen COL4A1, and lower activity with COL1A1. Catalyzes hydroxylation of the first Pro in Gly-Pro-Hyp sequences where Hyp is 4-hydroxyproline. Has no activity on substrates that have proline instead of 4-hydroxyproline in the third position (By similarity).
Images
WB analysis of LEPREL1(GB115118). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1500, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).