Recombinant Anti-Cytokeratin 19 antibody (Mouse mAb) servicebio GB15198-100
$50.00
In stock
SKU
GB15198-100
Product Information
| Protein full name | Keratin, type I cytoskeletal 19 |
| Synonyms | Cytokeratin-19, CK-19, Keratin-19, K19, Krt19, Krt1-19, 40 kDa keratin intermediate filament, K1CS, KRT19, CK19, K19, K1CS, keratin 19, Cytokeratin 19 |
| Uniprot ID | P08727 |
| Immunogen | KLH conjugated Synthetic peptide corresponding to Human Cytokeratin 19 |
| Isotype | IgG1, κ |
| Clone Number | SB35 |
| Predicted MW./Observed MW. | 44 kDa / 45 kDa |
| Purity | Affinity purification |
| Subcellular location | Cytoplasm |
Applications
| Applications: WB | Species: Human |
| Dilution: 1: 500-1: 1000 | Positive Tissue: HT-29, SW480, MCF7 |
| Applications: IHC/IF | Species: Human |
| Dilution: 1: 500-1: 1000 | Positive Tissue: breast cancer |
Background
The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis.
Images
WB analysis of Cytokeratin 19(GB15198). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 5000, RT, 1h.
IHC analysis of Cytokeratin 19(GB15198). Sample: Human breast cancer, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h.
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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