Anti-SIRT3 Rabbit pAb servicebio GB115590-50
$50.00
In stock
SKU
GB115590-50
Product Information
| Protein full name | NAD-dependent protein deacetylase sirtuin-3 |
| Synonyms | SIRT3, SIR2L3, sirtuin 3, hSIRT3, SIR2 like protein 3 |
| Uniprot ID | Q8R104 |
| Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse SIRT3 |
| Isotype | IgG |
| Predicted MW./Observed MW. | 36 kDa / 28 kDa |
| Purity | Affinity purification |
| Subcellular location | Mitochondrion, Cytoplasm |
Applications
| Applications: WB | Species: Mouse, Rat |
| Dilution: 1: 300-1: 400 | Positive Tissue: liver, lung |
| Applications: IHC/IF | Species: Mouse, Rat |
| Dilution: 1: 100-1: 200 | Positive Tissue: heart, spleen, skeletal muscle |
Background
NAD-dependent deacetylase sirtuin-3, mitochondrial also known as SIRT3 is member of the mammalian sirtuin family of proteins.NAD-dependent protein deacetylase. Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues. Known targets include ACSS1, IDH, GDH, PDHA1, SOD2, LCAD, SDHA and the ATP synthase subunit ATP5O. Contributes to the regulation of the cellular energy metabolism. Important for regulating tissue-specific ATP levels.
Images
WB analysis of SIRT3(GB115590). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 300, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.
IHC analysis of SIRT3(GB115590). Sample: Mouse heart, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Primary antibody: 1: 150, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.
IHC analysis of SIRT3(GB115590). Sample: Mouse skeletal muscle, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Primary antibody: 1: 150, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.
IHC analysis of SIRT3(GB115590). Sample: Mouse skeletal muscle, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Primary antibody: 1: 150, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.
IHC analysis of SIRT3(GB115590). Sample: Rat heart, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Primary antibody: 1: 150, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.
IHC analysis of SIRT3(GB115590). Sample: Rat skeletal muscle, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Primary antibody: 1: 150, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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