Anti-Phospho-S6 Ribosomal Protein (S240/S244) Rabbit pAb servicebio GB115636-100

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GB115636-100

 

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Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta-1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha-1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Integrins alpha-2/beta-1, alpha-3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha-10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin. Alpha-4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-5/beta-1 is a receptor for fibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin.

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Protein full nameSmall ribosomal subunit protein eS6
Synonyms40S ribosomal protein S6, Cell and organelle markers, Phosphoprotein NP33, Phospho-RPS6 (Ser240/244), Phospho-S6 Ribosomal Protein (Ser240/244), ribosomal protein S6, Ribosome Marker, RPS6, S6 ribosomal protein, p-S6RP, p-RPS6
ImmunogenKLH conjugated Synthetic phosphopeptide corresponding to Human RPS6 (S240/S244)
Uniprot IDP62753
IsotypeIgG
PurityAffinity purification
Subcellular locationNucleus, Cytoplasm
Predicted MW. / Observed MW.29 kDa / 32 kDa
ApplicationsSpecies
DilutionPositive Tissue
WBHuman, Mouse, Rat
1: 2000-1: 3000HeLa,NIH/3T3
IHC/IFHuman, Mouse, Rat
1: 300-1: 1000ovarian cancer , tonsil
StorageStore at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage BufferPBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.
WB analysis of Ribosomal Protein (S240/S244)(GB115636). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 2000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.IHC analysis of Ribosomal Protein (S240/S244)(GB115636). Sample: Rat spleen, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min. Primary antibody: 1: 300, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.
IHC analysis of Ribosomal Protein (S240/S244)(GB115636). Sample: Human tonsil, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min. Primary antibody: 1: 300, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. 

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