Anti-ATF-4 Rabbit pAb servicebio GB111137-100
$50.00
In stock
SKU
GB111137-100
Product Information
| Protein full name | Cyclic AMP-dependent transcription factor ATF-4 |
| Synonyms | cAMP-dependent transcription factor ATF-4, Activating transcription factor 4, C/EBP-related ATF, C/ATF, TaxREB67 homolog, Atf4 |
| Uniprot ID | Q06507 |
| Immunogen | Recombinant protein corresponding to Mouse ATF-4 |
| Isotype | IgG |
| Purity | Affinity purification |
| Subcellular location | Cell membrane, Cytoplasm, Nucleus |
Applications
| Applications: IHC/IF | Species: Human, Mouse, Rat |
| Dilution: 1: 1500-1: 3000 | Positive Tissue: bone, brain, breast cancer |
Background
Activating transcription factor 4 (tax-responsive enhancer element B67), also known as ATF4, is a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The protein is characterized as the cAMP-response element binding protein 2 (CREB-2) and belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. ATF4 transcription factor is also known to play role in osteoblast differentiation along with RUNX2 and osterix.
Images
IHC analysis of ATF-4(GB111137). Sample: Human colon, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min. Primary antibody: 1: 1600, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.
IHC analysis of ATF-4(GB111137). Sample: Mouse bone, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min. Primary antibody: 1: 1600, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.
IHC analysis of ATF-4(GB111137). Sample: Mouse brain, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min. Primary antibody: 1: 1600, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.
IHC analysis of ATF-4(GB111137). Sample: Mouse liver, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min. Primary antibody: 1: 1600, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.
IHC analysis of ATF-4(GB111137). Sample: Rat liver, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min. Primary antibody: 1: 1600, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.
IF analysis of ATF-4(GB111137)red. Sample: Human breast cancer, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 1500, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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