Anti-SNRNP200 Rabbit pAb servicebio GB112111-100
$50.00
In stock
SKU
GB112111-100
Product Information
| Protein full name | U5 small nuclear ribonucleoprotein 200 kDa helicase |
| Synonyms | BRR2 homolog, U5 snRNP-specific 200 kDa protein, U5-200KD, Snrnp200, Kiaa0788, BRR2, ASCC3L1, RP33 |
| Uniprot ID | Q6P4T2 |
| Immunogen | Recombinant protein corresponding to Mouse SNRNP200 |
| Isotype | IgG |
| Predicted MW./Observed MW. | 245 kDa / 245 kDa |
| Purity | Affinity purification |
| Subcellular location | Nucleus |
Applications
| Applications: WB | Species: Human, Mouse |
| Dilution: 1: 500-1: 1000 | Positive Tissue: colon |
| Applications: IHC/IF | Species: Mouse, Rat |
| Dilution: 1: 650-1: 1300 | Positive Tissue: ovary, testis |
Background
This gene encodes one of the U5 snRNP-specific proteins. This protein belongs to the DEXH-box family of putative RNA helicases. It is a core component of U4/U6-U5 snRNPs and appears to catalyze an ATP-dependent unwinding of U4/U6 RNA duplices. Mutations in this gene cause autosomal-dominant retinitis pigmentosa. Alternatively spliced transcript variants encoding different isoforms have been found, but the full-length nature of these variants has not been determined.
Images
WB analysis of SNRNP200(GB112111). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.
IF analysis of SNRNP200(GB112111)red. Sample: Mouse testis, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 650, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.
IF analysis of SNRNP200(GB112111)red. Sample: Rat ovary, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 650, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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