Anti-TIMP2 Rabbit pAb servicebio GB112670-100
$50.00
In stock
SKU
GB112670-100
Product Information
| Protein full name | Metalloproteinase inhibitor 2 |
| Synonyms | TIMP2, CSC-21K, DDC8, TIMP metallopeptidase inhibitor 2, CSC21K, Tissue Inhibitor of Metalloproteinase 2 |
| Uniprot ID | P16035 |
| Immunogen | KLH conjugated Synthetic peptide corresponding to Human TIMP2 |
| Isotype | IgG |
| Predicted MW./Observed MW. | 24 kDa / 24 kDa |
| Purity | Affinity purification |
| Subcellular location | Secreted |
Applications
| Applications: WB | Species: Human, Mouse, Rat |
| Dilution: 1: 500-1: 1000 | Positive Tissue: kidney, lung, placenta, pancreas |
| Applications: IHC/IF | Species: Mouse, Rat |
| Dilution: 1: 300-1: 800 | Positive Tissue: kidney, lung |
| Applications: ICC/IF | Species: Human, Mouse, Rat |
| Dilution: 1: 100-1: 400 | Positive Tissue: HeLa, NIH/3T3 |
Background
TIMP2 encoded by Timp2 gene are natural inhibitors of the matrix metalloproteinases (MMP), a group of peptidases involved in degradation of the extracellular matrix. In addition to an inhibitory role against metalloproteinases, it has a unique role among TIMP family members in its ability to directly suppress the proliferation of endothelial cells, which may be critical to the maintenance of tissue homeostasis by suppressing the proliferation of quiescent tissues in response to angiogenic factors, and by inhibiting protease activity in tissues undergoing remodelling of the extracellular matrix.
Images
WB analysis of TIMP2(GB112670). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.
WB analysis of TIMP2(GB112670). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.
WB analysis of TIMP2(GB112670). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.
IHC analysis of TIMP2(GB112670). Sample: Mouse placenta, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min. Primary antibody: 1: 300, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.
IHC analysis of TIMP2(GB112670). Sample: Rat kidney, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min. Primary antibody: 1: 300, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.
IHC analysis of TIMP2(GB112670). Sample: Rat lung, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min. Primary antibody: 1: 300, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.
IHC analysis of TIMP2(GB112670). Sample: Mouse kidney, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min. Primary antibody: 1: 300, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.
ICC analysis of TIMP2(GB112670)green. Sample: Hela, 4% PFA (G1101) 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 100, 4℃ overnight. Secondary antibody: 488 conjugated Goat Anti-Rabbit IgG (H+L) (GB25303), 1: 400, RT, 1h.
ICC analysis of TIMP2(GB112670)green. Sample: NIH-3T3, 4% PFA (G1101) 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 100, 4℃ overnight. Secondary antibody: 488 conjugated Goat Anti-Rabbit IgG (H+L) (GB25303), 1: 400, RT, 1h.
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
