Anti-COX IV Mouse mAb servicebio GB12250-100
$50.00
In stock
SKU
GB12250-100
Product Information
| Protein full name | Cytochrome c oxidase subunit 4 isoform 1, mitochondrial |
| Synonyms | Cytochrome c oxidase polypeptide IV, Cytochrome c oxidase subunit IV isoform 1, COX IV-1, Cox4i1, Cox4, Cox4a, COX4I1, COX4, COX4-1, COXIV, COXIV-1, COXIV-1, cytochrome c oxidase subunit 4I1 |
| Uniprot ID | P19783 |
| Immunogen | Recombinant protein corresponding to Mouse COX IV |
| Isotype | IgG2a, κ |
| Clone Number | 7D1E2 |
| Predicted MW./Observed MW. | 20 kDa / 17 kDa |
| Purity | Affinity purification |
| Subcellular location | Mitochondrion inner membrane |
Applications
| Applications: WB | Species: Mouse, Rat |
| Dilution: 1: 1000-1: 2000 | Positive Tissue: heart, liver, brain |
| Applications: IHC/IF | Species: Mouse, Rat |
| Dilution: 1: 1000-1: 2000 | Positive Tissue: brain, heart, kidney |
| Applications: ICC/IF | Species: Mouse |
| Dilution: 1: 50-1: 200 | Positive Tissue: NIH-3T3 |
Background
COX4I1, also named as COX4 and COXIV-1, belongs to the cytochrome c oxidase IV family. It is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. COX4I1 is a marker for mitochondria. It has two isoforms (isoform 1 and 2). Isoform 1(COX4I1) is ubiquitously expressed and isoform 2 is highly expressed in lung tissues. COX4I1 is commonly used as a loading control. This antibody was generated against full length COX4I1 protein and cross reacts with COX4I2.
Images
WB analysis of COX IV(GB12250). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 5000, RT, 1h.
IHC analysis of COX IV(GB12250). Sample: Mouse heart, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h.
IHC analysis of COX IV(GB12250). Sample: Mouse kidney, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h.
IHC analysis of COX IV(GB12250). Sample: Rat heart, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h.
IHC analysis of COX IV(GB12250). Sample: Rat kidney, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h.
IHC analysis of COX IV(GB12250). Sample: Rat liver, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h.
ICC analysis of COX IV(GB12250)green. Sample: NIH-3T3, 4% PFA (G1101) 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 50, 4℃ overnight. Secondary antibody: 488 conjugated Goat Anti-Mouse IgG (H+L) (GB25301), 1: 400, RT, 1h.
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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