Anti-YIPF4 Rabbit pAb servicebio GB113863-100
$50.00
In stock
SKU
GB113863-100
Product Information
| Protein full name | Protein YIPF4 |
| Synonyms | FinGER4, Nbla11189, Protein YIPF4, Yip1 domain family, member 4, YIP1 family member 4, YIPF4 |
| Uniprot ID | Q8C407 |
| Immunogen | Recombinant protein corresponding to Mouse YIPF4 |
| Isotype | IgG |
| Predicted MW./Observed MW. | 27 kDa / 27 kDa |
| Purity | Affinity purification |
| Subcellular location | Golgi apparatus |
Applications
| Applications: WB | Species: Human, Mouse, Rat |
| Dilution: 1: 1000-1: 2000 | Positive Tissue: spleen, placenta |
| Applications: IHC/IF | Species: Human, Mouse, Rat |
| Dilution: 1: 500-1: 1200 | Positive Tissue: colon, duodenum, heart |
Background
Involved in the maintenance of the Golgi structure.
Images
WB analysis of YIPF4(GB113863). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.
IHC analysis of YIPF4(GB113863). Sample: Mouse duodenum, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.
IHC analysis of YIPF4(GB113863). Sample: Rat duodenum, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.
IF analysis of YIPF4(GB113863)red. Sample: Human colon, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 600, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.
IF analysis of YIPF4(GB113863)red. Sample: Mouse colon, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 600, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.
IF analysis of YIPF4(GB113863)red. Sample: Rat colon, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 600, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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