Product Information
| Protein full name | ER membrane protein complex subunit 1 |
| Synonyms | Emc1, PSEC0263, RP23-371E13.1 |
| Uniprot ID | Q8C7X2 |
| Immunogen | Recombinant protein corresponding to Mouse KIAA0090 |
| Isotype | IgG |
| Purity | Affinity purification |
| Subcellular location | Endoplasmic reticulum membrane |
Applications
| Applications: IHC/IF | Species: Rat |
| Dilution: 1: 1000-1: 2000 | Positive Tissue: brain |
Background
Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins. Preferentially accommodates proteins with transmembrane domains that are weakly hydrophobic or contain destabilizing features such as charged and aromatic residues. Involved in the cotranslational insertion of multi-pass membrane proteins in which stop-transfer membrane-anchor sequences become ER membrane spanning helices. It is also required for the post-translational insertion of tail-anchored/TA proteins in endoplasmic reticulum membranes.
Images
IHC analysis of KIAA0090(GB115363). Sample: Rat brain, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).