Anti-Cofilin Rabbit pAb servicebio GB112496-50
$50.00
In stock
SKU
GB112496-50
Product Information
| Protein full name | Cofilin-1 |
| Synonyms | CFL1, CFL, HEL-S-15, Cofilin, Cofilin 1, 18 kDa phosphoprotein, p18, Cofilin, non-muscle isoform, Cfl1, HEL-S-15, epididymis secretory protein Li 15 |
| Uniprot ID | P18760 |
| Immunogen | Recombinant protein corresponding to Mouse Cofilin/KLH conjugated Synthetic peptide corresponding to Mouse Cofilin |
| Isotype | IgG |
| Predicted MW./Observed MW. | 19 kDa / 19 kDa |
| Purity | Affinity purification |
| Subcellular location | Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Nucleus |
Applications
| Applications: WB | Species: Human, Mouse, Rat |
| Dilution: 1: 500-1: 1000 | Positive Tissue: liver |
| Applications: IHC/IF | Species: Human, Mouse, Rat |
| Dilution: 1: 650-1: 1300 | Positive Tissue: liver, lung, lung cancer |
Background
Cofilin 1 (non-muscle; n-cofilin), also known as CFL1, is a member of the ADF/cofilin family. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus. One group reports that reelin signaling leads to serine3-phosphorylation of cofilin-1, and this interaction may play a role in the reelin-related regulation of neuronal migration.
Images
WB analysis of Cofilin(GB112496). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.
IF analysis of Cofilin(GB112496)red. Sample: Human lung cancer, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 650, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.
IF analysis of Cofilin(GB112496)red. Sample: Human ovarian cancer, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 650, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.
IF analysis of Cofilin(GB112496)red. Sample: Rat ovary, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 650, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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