Recombinant Rat CD48/Blast-1 Protein (Catalog#80286-R08H)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Rat CD48/Blast-1 (rR CD48/Blast-1; Catalog#80286-R08H; P10252; Met1-Arg216) and conjugated with APC under optimum conditions, the unreacted APC was removed.
Source
Monoclonal Rabbit IgG Clone #002
Purification
Protein A
Formulation
PBS solution containing 0.5% BSA and 0.09% sodium azide
Concentration
10 μl/Test, 0.1 mg/ml
Conjugate
APC
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze ! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.
Flow cytometric analysis of Rat CD48 expression on SD rat splenocytes. Cells were stained with APC-conjugated anti-Rat CD48. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Cluster of Differentiation 48 (CD48), also known as SLAMF2, BCM-1 and BLAST-1, is a GPI-linked protein belonging to the CD2 subfamily of immunoglobulin superfamily molecules. CD2 and 2B4 (CD244) are known ligands for CD48. CD48 protein is expressed on most lineage-committed hematopoietic cells but not on hematopoietic stem cells or multipotent hematopoietic progenitors. CD48 protein performs biological functions in a variety processes including adhesion, pathogen recognition, cellular activation, and cytokine regulation, and emerges as a critical effector molecule in immune responses.
Research Areas
Cancer Drug Targets
Tags
Cancer Immunotherapy
Co-inhibitory Immune Checkpoint Targets
Immune Checkpoint
Immune Checkpoint Detection: Antibodies
Immune Checkpoint Detection: ELISA Antibodies
Immune Checkpoint Detection: FCM Antibodies
Immune Checkpoint Detection: ICC Antibodies
Immune Checkpoint Detection: IHC Antibodies
Immune Checkpoint Detection: IP Antibodies
Immune Checkpoint Detection: WB Antibodies
Immune Checkpoint Proteins
Immune Checkpoint Targets
Immunotherapy
Targeted Therapy
References
Messmer B, et al. (2006) CD48 stimulation by 2B4 (CD244)-expressing targets activates human NK cells. J Immunol. 176(8): 4646-50
Milstein O, et al. (2008) Nanoscale increases in CD2-CD48-mediated intermembrane spacing decrease adhesion and reorganize the immunological synapse. J Biol Chem. 283(49): 34414-22.