| Catalog No. | Size | Price (USD) |
| 50134-R711-A-25 | 25 Tests | $44.43 |
| 50134-R711-A-100 | 100 Tests | $103.68 |
General Information
| Product name | Recombinant Anti-CD4 Antibody (APC), Rabbit Monoclonal |
| Validated applications | FCM (FAQ Protocol) |
| Species reactivity | Reacts with: Mouse |
| Specificity | Mouse CD4 |
| Immunogen | Recombinant Mouse CD4 Protein (Catalog#50134-M08H) |
| Preparation | This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD4 (rM CD4; Catalog#50134-M08H; NP_038516.1; Met1-Thr394) and conjugated with APC under optimum conditions, the unreacted APC was removed. |
| Source | Monoclonal Rabbit IgG Clone #711 |
| Purification | Protein A |
| Formulation | PBS solution containing 0.5% BSA and 0.03%ProClin300 |
| Concentration | 2 μl/Test, 0.1 mg/ml |
| Conjugate | APC |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze ! |
Synonyms: Anti-Cd4 Antibody; Anti-L3T4 Antibody; Anti-Ly-4 Antibody
Image / Experimental Information
| Image 1 Description | Flow cytometric analysis of Mouse CD4 expression on BABL/c splenocytes. Cells were stained with APC-conjugated anti-Mouse CD4. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
| Image 1 Alt Text | Mouse CD4 Flow Cytometry (FC) 23306 |
| Image 1 URL | Source image |
Background Information
| Full Name | CD4 molecule |
| Description | T-cell surface glycoprotein CD4, is a single-pass type I membrane protein. CD4 contains three Ig-like C2-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. CD4 is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells. The CD4 surface determinant, previously associated as a phenotypic marker for helper/inducer subsets of T lymphocytes, has now been critically identified as the binding/entry protein for human immunodeficiency viruses (HIV). The human CD4 molecule is readily detectable on monocytes, T lymphocytes, and brain tissues. All human tissue sources of CD4 bind radiolabeled gp120 to the same relative degree; however, the murine homologous protein, L3T4, does not bind the HIV envelope protein. CD4 is a co-receptor that assists the T cell receptor (TCR) to activate its T cell following an interaction with an antigen-presenting cell. Using its portion that resides inside the T cell, CD4 amplifies the signal generated by the TCR. CD4 interacts directly with MHC class II molecules on the surface of the antigen-presenting cell via its extracellular domain. The CD4 molecule is currently the object of intense interest and investigation both because of its role in normal T-cell function, and because of its role in HIV infection. CD4 is a primary receptor used by HIV-1 to gain entry into host T cells. HIV infection leads to a progressive reduction of the number of T cells possessing CD4 receptors. Viral protein U (VpU) of HIV-1 plays an important role in downregulation of the main HIV-1 receptor CD4 from the surface of infected cells. Physical binding of VpU to newly synthesized CD4 in the endoplasmic reticulum is an early step in a pathway leading to proteasomal degradation of CD4. Amino acids in both helices found in the cytoplasmic region of VpU in membrane-mimicking detergent micelles experience chemical shift perturbations upon binding to CD4, whereas amino acids between the two helices and at the C-terminus of VpU show no or only small changes, respectively. Paramagnetic spin labels were attached at three sequence positions of a CD4 peptide comprising the transmembrane and cytosolic domains of the receptor. VpU binds to a membrane-proximal region in the cytoplasmic domain of CD4. |
| Related Pathways |
|
| References |
|
title: Exploring the Targets of Gulao Yukang Pill in Rheumatoid Arthritis via the Hippo Signaling Pathway: An Integrated Network Pharmacology and Experimental Validation Study.
authors: Tao Wang; Gang Wang; ...; Ganggang Jiang
journal: International Journal of Rheumatic Diseases
date: 2025-07-29
pmid: 40725994
doi: 10.1111/1756-185x.70367
cited_by_count: 0
techniques: Enzyme-linked Immunosorbent Assay; Staining
bioz_score: 93
title: Discovery of an theranostic functional mAb for visualizing the sensitivity and effectiveness of PD-L1 checkpoint therapy.
authors: Jin Wang; Yanchen Li; ...; Yuanyuan Shan
journal: Bioorganic chemistry
date: 2024-11-22
pmid: 39603072
doi: 10.1016/j.bioorg.2024.107992
cited_by_count: 0
techniques: MTT Assay
bioz_score: 92
title: A bifunctional agent for efficient imaging of PD-L1 and antimelanoma activity.
authors: Jin Wang; Xiaoyan Pan; ...; Yuanyuan Shan
journal: Bioorganic chemistry
date: 2023-11-03
pmid: 37839142
doi: 10.1016/j.bioorg.2023.106912
cited_by_count: 0
techniques: MTT Assay; Nuclear Magnetic Resonance
bioz_score: 92
title: A universal influenza mRNA vaccine candidate boosts T-cell responses and reduces zoonotic influenza virus disease in ferrets
authors: van de Ven Koen; Lanfermeijer Josien; ...; de Jonge Jørgen
journal: bioRxiv
date: 2022-08-03
doi: 10.1101/2022.08.02.502529
cited_by_count: 1
techniques: FACS; Flow Cytometry; Staining
bioz_score: 92
title: Pathology and immunity after SARS-CoV-2 infection in male ferrets is affected by age and inoculation route
authors: van de Ven Koen; van Dijken Harry; ...; de Jonge Jørgen
journal: bioRxiv
date: 2021-06-30
doi: 10.1101/2021.06.30.450298
cited_by_count: 0
techniques: Incubation; Staining
bioz_score: 92
