E. coli acnB ELISA Kit sinobiological KIT13102
$714.00
In stock
SKU
KIT13102
| Catalog No. | Size | Price (USD) |
| KIT13102-1 | 1 Kit (96 Tests) | $740.55 |
General Information
| Product name | E. coli acnB ELISA Kit |
| Specificity | This assay recognizes both recombinant and natural AcnB. |
| Conjugate | HRP |
| Shipping | This ELISA Kit is shipped at ambient temperature. |
| Storage | Unopened Kit: Store at 2 - 8℃ Opened/Reconstituted Reagents: Please refer to CoA |
| Assay type | Solid Phase Sandwich ELISA (quantitative) |
| Sample type | Lysate |
| Sensitivity | 32.4 pg/mL |
| Assay range | 78.1-5000 pg/mL |
| Materials provided | 1. 96 well microplate coated with Capture Antibody 2. Detection Antibody conjugated to HRP 3. Standards 4. Wash Buffer Concentrate 5. Dilution Buffer Concentrate 6. Color Reagent A 7. Color Reagent B 8. Stop Solution |
| Product overview | This E. coli acnB ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative measurement of E. coli acnB protein in Lysate . It contains recombinant E. coli acnB, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use. |
Image / Experimental Information
| Image 1 Description | This standard curve is only for demonstration purposes. A standard curve should be generated for each assay. |
| Image 1 Alt Text | E. coli acnB ELISA Kit Standard Curve 118 |
| Image 1 URL | Source image |
Background Information
| Description | Escherichia coli contains two major aconitases (Acns), AcnA and AcnB. They are distantly related monomeric Fe-S proteins that contain different arrangements of four structural domains. acnA is specifically subject to SoxRS-mediated activation, whereas acnB encodes the major aconitase that is synthesized earlier in the growth cycle than AcnA. It is concluded that AcnB is the major citric acid cycle enzyme. Aconitate hydratase 2 (acnB) catalyzes the isomerization of citrate to isocitrate via cis-aconitate as well as the dehydration of 2-methylisocitrate to cis-2-methylaconitate, thus it functions as the major citric-acid-cycle enzyme during exponential growth. Escherichia coli acnB serves as either an enzymic catalyst or a mRNA-binding post-transcriptional regulator, depending on the status of its iron sulfur cluster. AcnB represents a large, distinct group of Gram-negative bacterial aconitases that have an altered domain organization relative to mitochondrial aconitase and other aconitases. |
| References |
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