Influenza A H9N2 (A/Hong Kong/1073/99) Hemagglutinin / HA ELISA Kit sinobiological KIT11229

$714.00
In stock
SKU
KIT11229
Catalog No.SizePrice (USD)
KIT11229-11 Kit (96 Tests)$740.55

General Information

Product nameInfluenza A H9N2 (A/Hong Kong/1073/99) Hemagglutinin / HA ELISA Kit
SpecificityThis assay recognizes both recombinant and natural Virus H9N2-H-99-HA.
ConjugateHRP
ShippingThis ELISA Kit is shipped at ambient temperature.
StorageUnopened Kit: Store at 2 - 8℃ Opened/Reconstituted Reagents: Please refer to CoA
Assay typeSolid Phase Sandwich ELISA (quantitative)
Sample typeRecombinant protein
Sensitivity13.62 pg/mL
Assay range78.13-5000 pg/mL
Materials provided1. 96 well microplate coated with Capture Antibody 2. Detection Antibody conjugated to HRP 3. Standards 4. Wash Buffer Concentrate 5. Dilution Buffer Concentrate 6. Color Reagent A 7. Color Reagent B 8. Stop Solution
Product overviewThis Influenza A H9N2 (A/Hong Kong/1073/99) Hemagglutinin / HA ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative measurement of H9N2 Hemagglutinin/HA protein in Recombinant protein . It contains recombinant H9N2 Hemagglutinin/HA, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.

Image / Experimental Information

Image 1 DescriptionThis standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
Image 1 Alt TextH9N2 HA ELISA Kit Standard Curve 18763
Image 1 URLSource image
Image 2 DescriptionThis standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
Image 2 Alt TextH9N2 HA ELISA Kit Specificity 18764
Image 2 URLSource image

Background Information

Full NameHemagglutinin
DescriptionThe influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
References
  1. White JM, Hoffman LR, Arevalo JH, et al. Attachment and entry of influenza virus into host cells. Pivotal roles of hemagglutinin. In Chiu W, Burnett RM, Garcea RL. Structural Biology of Viruses.1997
  2. Suzuki Y.Sialobiology of influenza: molecular mechanism of host range variation of influenza viruses. Biol. Pharm. Bull. 2005.
  3. Senne DA, Panigrahy B, Kawaoka Y, et al. Survey of the hemagglutinin (HA) cleavage site sequence of H5 and H7 avian influenza viruses: amino acid sequence at the HA cleavage site as a marker of pathogenicity potential. Avian Dis. 1996
  4. Donald J. Benton,Influenza hemagglutinin membrane anchor,PNAS,2018

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