| Catalog No. | Size | Price (USD) |
| KIT10119-1 | 1 Kit (96 Tests) | $414.71 |
| KIT10119-5 | 5 Kit (96 Tests) | $1,747.71 |
| KIT10119-48 | 48 Tests | $266.60 |
General Information
| Product name | Human IL-18 ELISA Kit |
| Specificity | This assay recognizes both recombinant and natural Human IL18. |
| Conjugate | HRP |
| Shipping | This ELISA Kit is shipped at ambient temperature. |
| Storage | Unopened Kit: Store at 2 - 8℃ Opened/Reconstituted Reagents: Please refer to CoA |
| Assay type | Solid Phase Sandwich ELISA (quantitative) |
| Sample type | Serum |
| Sensitivity | 3.28 pg/mL |
| Assay range | 31.25-2000 pg/mL |
| Materials provided | 1. 96 well microplate coated with Capture Antibody 2. Detection Antibody conjugated to HRP 3. Standards 4. Wash Buffer Concentrate 5. Dilution Buffer Concentrate 6. Color Reagent A 7. Color Reagent B 8. Stop Solution |
| Product overview | This Human IL-18 ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-18 protein in Serum . It contains recombinant Human IL-18, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use. |
| Interference | Preparations of the factors listed below at 50 ng/mL in a mid-range recombinant human IL18 control were assayed for interference. No significant interference was observed. |
Synonyms: IGIF ELISA Kit, Human; IL-18 ELISA Kit, Human; IL-1g ELISA Kit, Human; IL1F4 ELISA Kit, Human
Image / Experimental Information
| Image 1 Description | This standard curve is only for demonstration purposes. A standard curve should be generated for each assay. |
| Image 1 Alt Text | Human HPGD/15-PGDH ELISA Kit Standard Curve 18698 |
| Image 1 URL | Source image |
| Image 2 Description | This standard curve is only for demonstration purposes. A standard curve should be generated for each assay. |
| Image 2 Alt Text | Human HPGD/15-PGDH ELISA Kit Specificity 18699 |
| Image 2 URL | Source image |
Background Information
| Full Name | interleukin 18 |
| Description | Interleukin-18 (IL-18, also known as interferon-gamma inducing factor) is a proinflammatory cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other cells. This cytokine can induce the IFN-gamma production of T cells. The combination of IL-18 and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. IL-18 is an IL-1-like cytokine that requires cleavage with caspase-1 to become active, was found to increase IgE production in a CD4+ T cell -, IL-4- and STAT6-dependent fashion. IL-18 and T cell receptor-mediated stimulation could induce naive CD4+ T cells to develop into IL-4-producing cells in vitro. Thus, caspase-1 and IL-18 may be critical in the regulation of IgE production in vivo, providing a potential therapeutic target for allergic disorders. IL-18 production in primary synovial cultures and purified synovial fibroblasts was, in turn, upregulated by TNF-α and IL-1β, suggesting that monokine expression can feedback to promote Th1 cell development in the synovial membrane. Besides, synergistic combinations of IL-18, IL-12, and IL-15 may be of importance in sustaining both Th1 responses and monokine production in RA. |
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