Recombinant Human CD64/FCGR1 Protein (Catalog#10256-H08H)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human CD64/FCGR1 (rh CD64/FCGR1; Catalog#10256-H08H; NP_000557.1; Met1-Pro288) and conjugated with PerCP under optimum conditions, the unreacted PerCP was removed.
Source
Monoclonal Rabbit IgG Clone #401
Purification
Protein A
Formulation
PBS solution containing 0.5% BSA and 0.03%ProClin300
Concentration
10 μl/Test, 0.1 mg/ml
Conjugate
PerCP
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze !
Image / Experimental Information
Image 1 Description
Flow cytometric analysis of Human CD64 expression on human whole blood monocytes. Cells were stained with PerCP-conjugated anti-Human CD64. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable monocytes.
High affinity immunoglobulin gamma Fc receptor I, also known as FCGR1 and CD64, is an integral membraneglycoprotein and a member of the immunoglobulin superfamily. CD64 is a high affinity receptor for the Fc region of IgG gamma and functions in both innate and adaptive immune responses. Receptors that recognize the Fc portion of IgG function in the regulation of immune response and are divided into three classes designated CD64, CD32, and CD16. CD64 is structurally composed of asignal peptidethat allows its transport to the surface of a cell, threeextracellularimmunoglobulin domainsof the C2-type that it uses to bind antibody, a hydrophobictransmembrane domain, and a short cytoplasmic tail. CD64 isconstitutivelyfound on only macrophages and monocytes, but treatment of polymorphonuclear leukocyteswith cytokines likeIFNγandG-CSFcan induce CD64 expression on these cells. The inactivation of the mouse CD64 resulted in a wide range of defects in antibody Fc-dependent functions. Mouse CD64 is an early participant in Fc-dependent cell activation and in the development of immune responses.
Research Areas
Fc Receptors
References
Allen J.M., et al. ,(1988), Nucleotide sequence of three cDNAs for the human high affinity Fc receptor (FcRI). Nucleic Acids Res. 16:11824-11824.
Allen J.M., et al. , (1989), Isolation and expression of functional high-affinity Fc receptor complementary DNAs.Science 243:378-381.
Porges A.J., et al. ,(1992), Novel Fc gamma receptor I family gene products in human mononuclear cells.J. Clin. Invest. 90:2102-2109.
