Chinese hamster PLBL2 / PLBD2 ELISA Kit sinobiological KIT5A7664

$1,000.00
In stock
SKU
KIT5A7664
Catalog No.SizePrice (USD)
KIT5A7664-11 Kit (96 Tests)$1,036.78

General Information

Product nameChinese hamster PLBL2 / PLBD2 ELISA Kit
SpecificityRecognizes recombinant Chinese Hamster (CHO) PLBL2/PLBD2
ConjugateHRP
ShippingThis ELISA Kit is shipped at ambient temperature.
StorageUnopened Kit: Store at 2 - 8℃ Opened/Reconstituted Reagents: Please refer to CoA
Assay typeSolid Phase Sandwich ELISA (quantitative)
Sample typeCell supernatant
Sensitivity43.5 pg/mL
Assay range390.63-25000 pg/mL
Materials provided1. 96 well microplate coated with Capture Antibody 2. Detection Antibody conjugated to HRP 3. Standards 4. Wash Buffer Concentrate 5. Dilution Buffer Concentrate 6. Color Reagent A 7. Color Reagent B 8. Stop Solution
Product overviewThis Chinese hamster PLBL2 / PLBD2 ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative measurement of Chinese hamster PLBL2 / PLBD2 protein in Cell supernatant . It contains recombinant Chinese hamster PLBL2 / PLBD2, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.

Image / Experimental Information

Image 1 DescriptionThis standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
Image 1 Alt TextChinese hamster PLBL2 / PLBD2 ELISA Kit Standard Curve 37295
Image 1 URLSource image

Background Information

Full Namephospholipase B domain containing 2
DescriptionPLBD2 localizes to the lysosome, as its absence could plausibly lead to a serious yet unrecognized lysosomal storage disease. PLBD1 and PLBD2 are semi-orphans in the sense of being probable phospholipases of B class but with uncertain physiological substrates and thus functionalities. PLBD1 and PLBD2 constitute a small gene family (sequence homology class) within vertebrates though one that occurs expanded in some early diverging eukaryotes. PLBD2 presents a special difficulty in that a sequence of post-translational steps are apparently necessary for its activation. Without these, potential substrates can hardly be assayed. These steps include removal of the signal peptide, mannosylation appropriate to the lysosome targeting receptor, and self-catalytic proteolytic activation to expose the substrate binding site as this becomes appropriate.
References
  1. Morgan CP. et al., 2004), Biochem J. 382 (2): 441-9.
  2. Kim W. et al., 2011, Mol Cell. 44 (2): 325-40.
  3. Havugimana PC. et al., 2012. Cell. 150 (5): 1068-81.

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