| Catalog No. | Size | Price (USD) |
| 90857-C02H-UE-1 | 1 mg | $3,362.12 |
| 90857-C02H-UE-100 | 100 µg | $515.43 |
General Information
| Formulation | Supplied as sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| Endotoxin | < 0.5 EU per mg protein. |
| Shipping | It is supplied and shipped as liquid with dry ice. |
| Stability & Storage | Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Purity | ≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC. |
| Activity | 1.Immobilized Recombinant Cynomolgus CD3 epsilon/CD3e Protein (ECD, Fc Tag)(Cat: 90857-C02H-UE) at 2 μg/mL (100 μL/well) can bind biotinylated SP34, Human IgG1, the EC 50 is 1-3 ng/mL (Routinely tested). |
| Protein Construction | A DNA sequence encoding the Cynomolgus CD3E (Q95LI5-1) (Met1-Asp117) was expressed with the Fc region of human IgG1 at the C-terminus. |
| Expressed Host | HEK293 Cells |
| Species | Cynomolgus |
| Predicted N Terminal | Gln 22 |
| Molecule Mass | The recombinant Cynomolgus CD3E consists of 334 amino acids and predicts a molecular mass of 37.56 kDa. As a result of glycosylation, it migrates as an approximately 39-48 kDa band in SDS-PAGE under reducing conditions. |
Image / Experimental Information
| Image 1 Alt Text | Cynomolgus CD3 epsilon/CD3e Protein 45355 |
| Image 1 URL | Source image |
| Image 2 Alt Text | Cynomolgus CD3 epsilon/CD3e Protein 45487 |
| Image 2 URL | Source image |
| Image 3 Alt Text | Cynomolgus CD3 epsilon/CD3e Protein 48330 |
| Image 3 URL | Source image |
Background Information
| Full Name | CD3e molecule, epsilon (CD3-TCR complex) |
| Description | T-cell surface glycoprotein CD3 epsilon chain, also known as CD3E, is a single-pass type I membrane protein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. The CD3 epsilon subunit of the T cell receptor (TCR) complex contains two defined signaling domains, a proline-rich sequence and an immune tyrosine activation motifs (ITAMs), and this complex undergoes a conformational change upon ligand binding that is thought to be important for the activation of T cells. In the CD3 epsilon mutant mice, all stages of T cell development and activation that are TCR-dependent were impaired, but not eliminated, including activation of mature naïve T cells with the MHCII presented superantigen, staphylococcal enterotoxin B, or with a strong TCR cross-linking antibody specific for either TCR-Cbeta or CD3 epsilon. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. Homozygous mutations in CD3D and CD3E genes lead to a complete block in T-cell development and thus to an early-onset severe combined immunodeficiency phenotype. |
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| References |
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title: Bispecific anti-CD3 antibodies, bispecific activatable anti-CD3 antibodies, and methods of using the same
authors: Bryan Allen Irving; Sherry Lynn La Porte; ...; Jennifer Hope Richardson
date: 2023-10-31
cited_by_count: 0
techniques: Concentration Assay; Enzyme-linked Immunosorbent Assay; Incubation; Purification; Recombinant
bioz_score: 91
