| Catalog No. | Size | Price (USD) |
| 40017-VNAHC4-1000 | 1000 U | $414.71 |
General Information
| Formulation | Lyophilized from sterile PBS, 0.6% Triton X-100, 7% Trehalose, 6% Mannitol, pH 7.4 Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| Endotoxin | Please contact us for more information. |
| Shipping | In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
| Stability & Storage | Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Reconstitution | To find reconstitution info, click on 'COA' at the top of the page, enter the lot number and product size for the lot-specific COA. |
| Activity | Measured by its ability to cleave a fluorogenic substrate, 2’-(4-Methylumbelliferyl)-α-D-N-acetylneuraminic acid. One unit is defined as the amount of enzyme required to cleave 1 nmole of 2'-(4-Methylumbelliferyl)-α-D-N-acetylneuraminic acid per minute at pH 7.5 at 37℃. |
| Protein Construction | A DNA sequence encoding the Influenza A virus (A/Babol/36/2005 (H3N2)) neuraminidase (ACN50232.1) (His 36-Pro 459) was expressed, Collected cell lysates are validated for neuraminidase activity. There is an amino acid change from Histidine to Tyrosine (H274Y mutation) in NA / Neuraminidase. |
| Accession# | ACN50232.1 |
| Expressed Host | HEK293 Cells |
| Species | H3N2 |
| Molecule Mass | The influenza H3N2 virus Neuraminidase comprises 424 amino acids. |
Background Information
| Description | Neuraminidases are enzymes that cleave sialic acid groups from glycoproteins. Influenza neuraminidase is a type of neuraminidase found on the surface of influenza viruses that enables the virus to be released from the host cell. Influenza neuraminidase is composed of four identical subunits arranged in a square. It is normally attached to the virus surface through a long protein stalk. The active sites are in a deep depression on the upper surface. They bind to polysaccharide chains and clip off the sugars at the end. The surface of neuraminidase is decorated with several polysaccharide chains that are similar to the polysaccharide chains that decorate our cell surface proteins. Neuraminidase (NA) and hemagglutinin (HA) are major membrane glycoproteins found on the surface of the influenza virus. Hemagglutinin binds to the sialic acid-containing receptors on the surface of host cells during initial infection and at the end of an infectious cycle. Neuraminidase, on the other hand, cleaves the HA-sialic acid bondage from the newly formed virions and the host cell receptors during budding. Neuraminidase thus is described as a receptor-destroying enzyme that facilitates virus release and efficient spread of the progeny virus from cell to cell. Influenza antibody and influenza antibodies are very important research tools for influenza diagnosis, influenza vaccine development, and anti-influenza virus therapy development. The monoclonal or polyclonal antibody can be raised with protein based antigen or peptide-based antigen. Antibodies raised with protein-based antigen could have better specificity and/or binding affinity than antibodies raised with peptide based antigen, but the cost associated with the recombinant protein antigen is usually higher. Anti-influenza virus hemagglutinin (HA) monoclonal antibody or polyclonal antibody can be used for ELISA assay, western blotting detection, Immunohistochemistry (IHC), flow cytometry, neutralization assay, hemagglutinin inhibition assay, and early diagnosis of influenza viral infection. Sino Biological has developed state-of-the-art monoclonal antibody development technology platforms: mouse monoclonal antibody and rabbit monoclonal antibody. Our rabbit monoclonal antibody platform is one of a kind and offers some unique advantages over mouse monoclonal antibodies, such as high affinity, low cross-reactivity with rabbit polyclonal antibodies. |
| References |
|
title: Preparation of a thioxoimidazolidin-linked sialoside BSA conjugate for the inhibition of influenza virus.
authors: Haijuan Qin; Zhiwei Huang; ...; Yang Yang
journal: Carbohydrate Research
date: 2024-10-09
pmid: 39405815
doi: 10.1016/j.carres.2024.109287
cited_by_count: 0
techniques: Control; Mass Spectrometry; Mutagenesis; Recombinant; SPR Assay
bioz_score: 92
title: A Zanamivir-protein conjugate mimicking mucin for trapping influenza virion particles and inhibiting neuraminidase activity.
authors: Mingming Xue; Ang Deng; ...; Yang Yang
journal: International Journal of Biological Macromolecules
date: 2024-08-08
pmid: 38955298
doi: 10.1016/j.ijbiomac.2024.133564
cited_by_count: 2
techniques: Electron Microscopy; Mutagenesis; Recombinant; SPR Assay; Titration; Transmission Assay; Transmission Electron Microscopy
bioz_score: 92
title: Chemoenzymatic synthesis of sialyl-α2,3-lactoside-functionalized BSA conjugate inhibits influenza infection.
authors: Mingming Xue; Lintongqing Tan; ...; Yang Yang
journal: European Journal of Medicinal Chemistry
date: 2024-06-26
pmid: 38968785
doi: 10.1016/j.ejmech.2024.116633
cited_by_count: 1
techniques: Centrifugation; Chromatography; Column Chromatography; Control; Mutagenesis; Purification; Recombinant
bioz_score: 92
title: Preparation of eicosavalent triazolylsialoside-conjugated human serum albumin as a dual hemagglutinin/neuraminidase inhibitor and virion adsorbent for the prevention of influenza infection.
authors: Yang Li; Han-Yu Liu; ...; Yang Yang
journal: Carbohydrate Research
date: 2023-08-30
pmid: 37586142
doi: 10.1016/j.carres.2023.108918
cited_by_count: 4
techniques: Column Chromatography; Mutagenesis; Recombinant; SPR Assay; Staining; Thin Layer Chromatography; Virus
bioz_score: 92
title: Synthetic pentatrideca-valent triazolylsialoside inhibits influenza virus hemagglutinin/neuraminidase and aggregates virion particles.
authors: Zhiqi Lao; Yang Li; ...; Yang Yang
journal: European Journal of Medicinal Chemistry
date: 2023-08-22
pmid: 37467617
doi: 10.1016/j.ejmech.2023.115578
cited_by_count: 6
techniques: Column Chromatography; Control; Mutagenesis; Recombinant; SPR Assay; Staining; Thin Layer Chromatography; Virus
bioz_score: 92
title: Discovery of novel “Dual-site” binding oseltamivir derivatives as potent influenza virus neuraminidase inhibitors
authors: Wei Ai; Jian Zhang; ...; Xinyong Liu
journal: European Journal of Medicinal Chemistry
date: 2020-04-01
doi: 10.1016/j.ejmech.2020.112147
cited_by_count: 0
techniques: Incubation; Mutagenesis; Virus
bioz_score: 93
title: Anti-Influenza Virus (H5N1) Activity Screening on the Phloroglucinols from Rhizomes of Dryopteris crassirhizoma
authors: Juan Wang; Yan-Tao Yan; ...; Zeng-Ping Gao
journal: Molecules
date: 2017-03-08
doi: 10.3390/molecules22030431
cited_by_count: 33
techniques: Purification
bioz_score: 93
title: Discovery of N-Substituted Oseltamivir Derivatives as Potent and Selective Inhibitors of H5N1 Influenza Neuraminidase
authors: Yuanchao Xie; Dongqing Xu; ...; Wenfang Xu
journal: Journal of Medicinal Chemistry
date: 2014-10-08
doi: 10.1021/jm500892k
cited_by_count: 63
techniques: Enzyme Inhibition Assay; Inhibition
bioz_score: 93
