WB (FAQ Protocol), ELISA (FAQ Protocol), IP (FAQ Protocol)
Species reactivity
Reacts with: Rat
Specificity
Rat Adenylate Kinase 1
Immunogen
Recombinant Rat AK1 / Adenylate kinase 1 Protein (Catalog#80583-R07E)
Preparation
Produced in rabbits immunized with purified, recombinant Rat AK1 / Adenylate kinase 1 (rR AK1 / Adenylate kinase 1; Catalog#80583-R07E; NP_077325.2; Met1-Lys194). AK1 / Adenylate kinase 1 specific IgG was purified by Rat AK1 / Adenylate kinase 1 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
Synonyms: Anti-Ak 1 Antibody; Anti-Ak1 Antibody
Image / Experimental Information
Image 1 Description
Anti-AK1 rabbit polyclonal antibody at 1:500 dilution Lane A: Rat Heart tissue lysate Lane B: Rat Brain tissue lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:22 kDa Observed band size:22 kDa
Anti-AK1 rabbit polyclonal antibody at 1:500 dilution Lane A: Rat Heart tissue lysate Lane B: Rat Brain tissue lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:22 kDa Observed band size:22 kDa
Image 2 Alt Text
Rat Adenylate Kinase 1 Immunoprecipitation(IP) 20227
The adenylate kinase (AK) isoforms network plays an important role in the intracellular energy transfer processes, the maintenance of energy homeostasis, and it is a major player in AMP metabolic signaling circuits in some highly-differentiated cells. Three other biomarkers (AK1, CLIC1, and SOD1) were significantly increased in both adenoma and early colorectal cancer patient plasma samples and in plasma from colorectal cancer mouse models at preclinical stages compared with controls.Which can contribute to the detection of early-stage colorectal cancer and adenomas.
References
Lu Q, et al. (1996) Adenylate kinase complements nucleoside diphosphate kinase deficiency in nucleotide metabolism. Proc Natl Acad Sci. 93 (12): 5720-5.
Dzeja P, et al. (2009) Adenylate kinase and AMP signaling networks: Metabolic monitoring, signal communication and body energy sensing. Int J Mol Sci. 10 (4): 1729-72.
Lagresle-Peyrou C, et al. (2008) Human adenylate kinase 2 deficiency causes a profound hematopoietic defect associated with sensorineural deafness. Nature Genetics. 41: 106 - 11.