| Catalog No. | Size | Price (USD) |
| 11684-RP01-20 | 20 µL | $88.87 |
| 11684-RP01-100 | 100 µL | $251.64 |
| 11684-RP01-200 | 200 µL | $370.28 |
General Information
| Product name | Influenza A H1N1 Hemagglutinin / HA Antibody, Rabbit PAb |
| Validated applications | WB (FAQ Protocol), ELISA (FAQ Protocol) |
| Application notes | IHC, FCM, IF, IP et al. applications haven't been validated. (Antibody's applications haven't been validated with corresponding virus positive samples. Optimal concentrations/dilutions should be determined by the end user.) |
| Specificity | H1N1 (A/Puerto Rico/8/1934) HA |
| Immunogen | Recombinant H1N1 HA Protein (Catalog#11684-V08H) |
| Preparation | Produced in rabbits immunized with purified, recombinant Influenza A H1N1 (A/Puerto Rico/8/1934) hemagglutinin (Catalog#11684-V08H; ABD77675.1; Met 1-Gln 528). Total IgG was purified by Protein A affinity chromatography. |
| Source | Polyclonal Rabbit IgG |
| Purification | Protein A |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Background Information
| Full Name | Hemagglutinin |
| Description | The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity. |
| References |
|
title: Real-Time Dissection of the Exosome Pathway for Influenza Virus Infection.
authors: Jian Ao; Ai-Xin Ma; ...; Dai-Wen Pang
journal: ACS Nano
date: 2024-02-07
pmid: 38270127
doi: 10.1021/acsnano.3c11309
cited_by_count: 6
techniques: Cell Culture; Immunofluorescence; Modification; Polymerase Chain Reaction; Virus; Western Blot
bioz_score: 95
title: High salt activates p97 to reduce host antiviral immunity by restricting Viperin induction.
authors: Yukang Yuan; Ying Miao; ...; Hui Zheng
journal: EMBO Reports
date: 2022-03-14
pmid: 34779558
doi: 10.15252/embr.202153466
cited_by_count: 11
techniques: Ubiquitin Proteomics; Western Blot
bioz_score: 95
title: Protein Disulfide Isomerase A3 Regulates Influenza Neuraminidase Activity and Influenza Burden in the Lung
authors: Nicolas Chamberlain; Mona Ruban; ...; Andreas Burkovski
journal: International Journal of Molecular Sciences
date: 2022-01-19
pmid: 35162999
doi: 10.3390/ijms23031078
pmcid: 8834910
cited_by_count: 8
techniques: DC Protein Assay; Expressing; Software; Western Blot
bioz_score: 95
title: MARCH8 inhibits influenza A virus infection by targeting viral M2 protein for ubiquitination-dependent degradation in lysosomes
authors: Xiaoman Liu; Fengwen Xu; ...; Fei Guo
journal: Nature Communications
date: 2021-07-20
pmid: 34285233
doi: 10.1038/s41467-021-24724-2
pmcid: 8292393
cited_by_count: 51
techniques: Construct; Mutagenesis; Plasmid Preparation; Western Blot
bioz_score: 95
title: A new and simplified anion exchange chromatographic process for the purification of cell-grown influenza A H1N1 virus
authors: Duy Tien Ta; Kai Ling Chu; ...; Wei Zhang
journal: Separation and Purification Technology
date: 2021-05-01
doi: 10.1016/j.seppur.2021.118412
cited_by_count: 3
techniques: Incubation; Purification; Staining; Western Blot
bioz_score: 95
title: Zwitterionic Polydopamine/Protein G Coating for Antibody Immobilization: Toward Suppression of Nonspecific Binding in Immunoassays.
authors: Jihyun Byun; Soojeong Cho; ...; Taejoon Kang
journal: ACS Applied Bio Materials
date: 2020-06-15
pmid: 35025233
doi: 10.1021/acsabm.0c00264
cited_by_count: 6
techniques: Microscopy
bioz_score: 95
title: Exchange of amino acids in the H1-haemagglutinin to H3 residues is required for efficient influenza A virus replication and pathology in Tmprss2 knock-out mice
authors: Ruth L. O. Lambertz; Jan Pippel; ...; Klaus Schughart
journal: The Journal of general virology
date: 2018-09-01
pmid: 30084768
doi: 10.1099/jgv.0.001128
pmcid: 6230768
cited_by_count: 12
techniques: Bicinchoninic Acid Protein Assay; Derivative Assay; Generated; Imaging; Incubation; Infection; Membrane; Produced; Recombinant; SDS Page; Software; Staining; Virus; Western Blot
bioz_score: 95
title: Enterokinase Enhances Influenza A Virus Infection by Activating Trypsinogen in Human Cell Lines
authors: Hideki Hayashi; Yoshinao Kubo; ...; Toshifumi Matsuyama
journal: Frontiers in cellular and infection microbiology
date: 2018-03-23
pmid: 29629340
doi: 10.3389/fcimb.2018.00091
pmcid: 5876233
cited_by_count: 11
techniques: SDS Page; Western Blot
bioz_score: 95
title: Vaccination against Influenza with Recombinant Hemagglutinin Expressed by Schizochytrium sp. Confers Protective Immunity
authors: Anne-Cécile V. Bayne; David Boltz; ...; Paul G. Thomas
journal: PLoS ONE
date: 2013-04-23
pmid: 23626728
doi: 10.1371/journal.pone.0061790
pmcid: 3634000
cited_by_count: 47
techniques: Mass Spectrometry; Membrane; Nucleic Acid Electrophoresis; Sequencing; Staining; Virus; Western Blot
bioz_score: 95
