| Catalog No. | Size | Price (USD) |
| 40569-V07H-50 | 50 µg | $380.64 |
| 40569-V07H-100 | 100 µg | $765.73 |
General Information
| Formulation | Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| Endotoxin | < 1.0 EU per μg protein as determined by the LAL method. |
| Shipping | In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
| Stability & Storage | Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Reconstitution | To find reconstitution info, click on 'COA' at the top of the page, enter the lot number and product size for the lot-specific COA. |
| Purity | > 95 % as determined by SDS-PAGE. |
| Activity | Testing in progress |
| Protein Construction | A DNA sequence encoding the Influenza A virus (A/Hong Kong/4801/2014 (H3N2)) neuraminidase (translated amino acids of EPI675797) (His36-Ile469) was expressed with a polyhistidine tag at the N-terminus. |
| Accession# | EPI675797 |
| Expressed Host | HEK293 Cells |
| Species | H3N2 |
| Predicted N Terminal | His |
| Molecule Mass | The recombinant Influenza A virus (A/Hong Kong/4801/2014 (H3N2)) NA consists of 453 amino acids and predicts a molecular mass of 50.5 kDa. |
Image / Experimental Information
| Image 1 Alt Text | H3N2 Neuraminidase / NA Protein 19516 |
| Image 1 URL | Source image |
Background Information
| Description | Neuraminidases are enzymes that cleave sialic acid groups from glycoproteins. Influenza neuraminidase is a type of neuraminidase found on the surface of influenza viruses that enables the virus to be released from the host cell. Influenza neuraminidase is composed of four identical subunits arranged in a square. It is normally attached to the virus surface through a long protein stalk. The active sites are in a deep depression on the upper surface. They bind to polysaccharide chains and clip off the sugars at the end. The surface of neuraminidase is decorated with several polysaccharide chains that are similar to the polysaccharide chains that decorate our cell surface proteins. Neuraminidase (NA) and hemagglutinin (HA) are major membrane glycoproteins found on the surface of the influenza virus. Hemagglutinin binds to the sialic acid-containing receptors on the surface of host cells during initial infection and at the end of an infectious cycle. Neuraminidase, on the other hand, cleaves the HA-sialic acid bondage from the newly formed virions and the host cell receptors during budding. Neuraminidase thus is described as a receptor-destroying enzyme that facilitates virus release and efficient spread of the progeny virus from cell to cell. Influenza antibody and influenza antibodies are very important research tools for influenza diagnosis, influenza vaccine development, and anti-influenza virus therapy development. The monoclonal or polyclonal antibody can be raised with protein based antigen or peptide-based antigen. Antibodies raised with protein-based antigen could have better specificity and/or binding affinity than antibodies raised with peptide based antigen, but the cost associated with the recombinant protein antigen is usually higher. Anti-influenza virus hemagglutinin (HA) monoclonal antibody or polyclonal antibody can be used for ELISA assay, western blotting detection, Immunohistochemistry (IHC), flow cytometry, neutralization assay, hemagglutinin inhibition assay, and early diagnosis of influenza viral infection. Sino Biological has developed state-of-the-art monoclonal antibody development technology platforms: mouse monoclonal antibody and rabbit monoclonal antibody. Our rabbit monoclonal antibody platform is one of a kind and offers some unique advantages over mouse monoclonal antibodies, such as high affinity, low cross-reactivity with rabbit polyclonal antibodies. |
| References |
|
title: Influenza mRNA vaccines
authors: Edith Jasny; Susanne Rauch; ...; Karen Slobod
date: 2025-12-30
cited_by_count: 0
techniques: Construct; Expressing; Flow Cytometry; Software; Staining; Transfection
bioz_score: 93
title: Binding antibody titers against the hemagglutinin and neuraminidase correlate with protection against medically attended influenza A and B disease.
authors: Marios Koutsakos; Arnold Reynaldi; ...; Philip A Mudd
journal: Journal of virology
date: 2025-05-13
pmid: 40358209
cited_by_count: 1
techniques: Blocking Assay; Clinical Proteomics; Enzyme-linked Immunosorbent Assay; Negative Control; Virus
bioz_score: 93
title: Development and characterization of an antibody that recognizes influenza virus N1 neuraminidases
authors: Nan Chen; Renxi Wang; ...; Victor C. Huber
journal: PLoS ONE
date: 2024-05-09
pmid: 38723016
doi: 10.1371/journal.pone.0302865
pmcid: 11081314
cited_by_count: 1
techniques: Construct; Derivative Assay; Recombinant
bioz_score: 90
title: Influenza a Neuraminidase-Based Bivalent mRNA Vaccine Induces Th1-Type Immune Response and Provides Protective Effects in Mice
authors: Mingyang Li; Mengyuan Liu; ...; Ralph A. Tripp
journal: Vaccines
date: 2024-03-13
pmid: 38543934
doi: 10.3390/vaccines12030300
pmcid: 10974139
cited_by_count: 5
techniques: Activity Assay; Blocking Assay; Concentration Assay; Enzyme-linked Immunosorbent Assay; Incubation
bioz_score: 92
title: Generation and characterization of monoclonal antibodies against the hemagglutinin of H3N2 influenza A viruses.
authors: Fan Yang; Linwei Zhu; ...; Lanjuan Li
journal: Virus research
date: 2022-06-03
pmid: 35595011
doi: 10.1016/j.virusres.2022.198815
cited_by_count: 6
techniques: Modification; Purification; Virus
bioz_score: 92
title: Robust and prototypical immune responses toward influenza vaccines in the high-risk group of Indigenous Australians
authors: Luca Hensen; Thi H. O. Nguyen; ...; Katherine Kedzierska
journal: Proceedings of the National Academy of Sciences of the United States of America
date: 2021-10-12
pmid: 34607957
doi: 10.1073/pnas.2109388118
pmcid: 8522271
cited_by_count: 5
techniques: MicroChIP Assay; Multiplex Assay; Produced; Purification
bioz_score: 90
title: Memory B Cell Activation, Broad Anti-influenza Antibodies, and Bystander Activation Revealed by Single-Cell Transcriptomics
authors: Felix Horns; Cornelia L. Dekker; Stephen R. Quake
journal: Cell Reports
date: 2021-02-03
pmid: 31968262
doi: 10.1016/j.celrep.2019.12.063
pmcid: 7891556
cited_by_count: 159
techniques: Cell Isolation; Multiplex Assay; Recombinant; Sequencing; Software
bioz_score: 90
title: Use of traditional serological methods and oral fluids to assess immunogenicity in children aged 2–16 years after successive annual vaccinations with LAIV
authors: Katja Hoschler; Sunil Maharjan; ...; Maria Zambon
journal: Vaccine
date: 2020-03-10
pmid: 32070679
doi: 10.1016/j.vaccine.2020.02.024
pmcid: 7054836
cited_by_count: 7
techniques: Cell Culture; Enzyme-linked Immunosorbent Assay; Recombinant; Sterility; Vaccines; Virus
bioz_score: 90
title: Transcriptional program of memory B cell activation, broadly binding anti-influenza antibodies, and bystander activation after vaccination revealed by single-cell transcriptomics
authors: Horns Felix; Dekker Cornelia L.; Quake Stephen R.
journal: bioRxiv
date: 2019-07-22
doi: 10.1101/709337
cited_by_count: 0
techniques: Purification
bioz_score: 90
title: Antenatal Influenza A-Specific IgA, IgM, and IgG Antibodies in Mother’s Own Breast Milk and Donor Breast Milk, and Gastric Contents and Stools from Preterm Infants
authors: Veronique Demers-Mathieu; Robert K. Huston; ...; David C. Dallas
journal: Nutrients
date: 2019-07-11
pmid: 31336756
doi: 10.3390/nu11071567
pmcid: 6682892
cited_by_count: 29
techniques: Incubation; Sample Prep
bioz_score: 90
