| Catalog No. | Size | Price (USD) |
| 10374-H27H1-B-20 | 20 µg | $333.25 |
| 10374-H27H1-B-50 | 50 µg | $669.46 |
| 10374-H27H1-B-100 | 100 µg | $835.35 |
General Information
| Formulation | Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| Biotin/Protein Ratio | 0.7-1 as determined by the HABA assay. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Shipping | In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
| Stability & Storage | Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Reconstitution | To find reconstitution info, click on 'COA' at the top of the page, enter the lot number and product size for the lot-specific COA. |
| Purity | ≥ 95 % as determined by SDS-PAGE, ≥ 95 % as determined by SEC-HPLC, ≥ 95% as determined by SEC-MALS(Routinely tested). |
| Activity | 1. Loaded Recombinant Human CD32a/FCGR2A Protein(H167,His&AVI Tag),Biotinylated (Cat: 10374-H27H1-B) on SA Biosensor, can bind anti-CD20(Research grade Rituximab Biosimilar) with an affinity constant of 0.53 μM as determined in BLI assay (Sartorius Octet RED384) (QC tested). 2. Recombinant Human CD32a/FCGR2A Protein(H167,His&AVI Tag),Biotinylated (Cat: 10374-H27H1-B) captured on SA chip, can bind anti-CD20(Research grade Rituximab Biosimilar) with an affinity constant of 0.568 μM as determined in an SPR assay (Biacore 8K) (Routinely tested). |
| Protein Construction | A DNA sequence encoding the extracellular domain (Met1-Met210) of human FCGR2A (P12318-1, with Natural variant H167) was fused with a C-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. |
| Accession# | P12318-1 |
| Expressed Host | HEK293 Cells |
| Species | Human |
| Predicted N Terminal | Gln 34 |
| Molecule Mass | The recombinant human CD32a consists of 213 amino acids after removal of the signal peptide and predicts a molecular mass of 23.7 KDa. It migrates as an approximately 33 KDa band due to glycosylation by SDS-PAGE under reducing conditions. The molecular weight of this protein is around 20-38 kDa verified by SEC-MALS (Routinely tested). |
Synonyms: CD32 Protein, Human; CD32A Protein, Human; CDw32 Protein, Human; Fc-gamma-RIIa Protein, Human; FCG2 Protein, Human; FcgammaRIIa Protein, Human; FcGR Protein, Human; FCGR2 Protein, Human; FCGR2A1 Protein, Human; IGFR2 Protein, Human
Image / Experimental Information
| Image 1 Alt Text | Human CD32a/FCGR2A/Fc gamma RIIA Protein 8740 |
| Image 1 URL | Source image |
| Image 2 Alt Text | Human CD32A Protein 35432 |
| Image 2 URL | Source image |
| Image 3 Alt Text | Human CD32A Protein 35476 |
| Image 3 URL | Source image |
| Image 4 Alt Text | Human CD32A Protein 35823 |
| Image 4 URL | Source image |
| Image 5 Alt Text | Human CD32A/FCGR2A Protein 42078 |
| Image 5 URL | Source image |
Background Information
| Full Name | Fc fragment of IgG receptor IIa |
| Research Areas |
|
title: FCRL5 is a fucose-sensitive IgG-Fc receptor with binding properties distinct from classical Fcγ receptors
authors: van der Hoeven Nick; Holborough-Kerkvliet Miles D; ...; Rispens Theo
journal: bioRxiv
date: 2026-07-07
doi: 10.64898/2026.07.01.735886
cited_by_count: 0
techniques: Recombinant
bioz_score: 94
title: Long-term cross-variant Fc-mediated immune responses against SARS-CoV-2 induced by a heterologous adenoviral/inactivated virus prime-boost vaccination strategy.
authors: Doyoung Kim; Jung Hyuk Lee; ...; Manki Song
journal: NPJ vaccines
date: 2026-05-22
pmid: 42168193
doi: 10.1038/s41541-026-01483-z
cited_by_count: 0
techniques: Cell Culture; Incubation; Modification; Neutralization
bioz_score: 94
title: A hexamerization-enhanced, Fc-silenced agonistic CD27 antibody amplifies T-cell effector functions as single agent and in combination with PD-1 blockade
authors: Işıl Altıntaş; Kristina B. Nürmberger; ...; Uğur Şahin
journal: Scientific Reports
date: 2025-07-24
pmid: 40707623
cited_by_count: 0
bioz_score: 93
title: Antibody or chimeric antigen receptor which targets Claudin 18.2
authors: Deyong Song; Li Zhou; ...; Zhenfei Ning
date: 2025-05-27
cited_by_count: 0
techniques: Modification
bioz_score: 94
title: Impact of Monoclonal Antibody Aggregates on Effector Function Characterization
authors: Wendy J. Walton; Shousong Jason Zhang; ...; Yingmei Gu
journal: Antibodies
date: 2025-04-02
pmid: 40265412
doi: 10.3390/antib14020031
pmcid: 12015860
cited_by_count: 2
techniques: Activation Assay; Binding Assay; Cell Based Assay; Expressing; Injection; Isolation; Luciferase; Reporter Assay; SPR Assay; Software; Standard Deviation
bioz_score: 94
title: Dose-dependent serological profiling of AdCLD-CoV19-1 vaccine in adults
authors: Jung Hyuk Lee; Yuna Shin; ...; Andrew Pekosz
journal: mSphere
date: 2025-01-01
pmid: 39723823
pmcid: 11774024
cited_by_count: 1
techniques: Binding Assay; Incubation; MANN-WHITNEY; Variant Assay
bioz_score: 94
title: A Novel Dual-Fc Bispecific Antibody with Enhanced Fc Effector Function.
authors: Fulai Zhou; Yinyin Ben; ...; Mark L Chiu
journal: Biochemistry
date: 2024-04-17
pmid: 38426700
doi: 10.1021/acs.biochem.3c00481
cited_by_count: 1
techniques: Binding Assay; Fluorescence; Transferring
bioz_score: 94
title: NNV024, a novel humanized anti-CD37 antibody with enhanced ADCC and prolonged plasma half-life in human FcRn transgenic mice for treatment of NHL
authors: Generalov Roman; Fiorito Elisa; ...; Dahle Jostein
journal: bioRxiv
date: 2023-07-12
doi: 10.1101/2023.07.12.548667
cited_by_count: 0
techniques: Binding Assay; Blocking Assay; Concentration Assay; Enzyme-linked Immunosorbent Assay; Incubation
bioz_score: 94
title: FcγRIIA-specific DARPins as novel tools in blood cell analysis and platelet aggregation
authors: Vanessa Riechert; Sascha Hein; ...; Christian J. Buchholz
journal: The Journal of Biological Chemistry
date: 2023-04-24
pmcid: 10209026
cited_by_count: 0
techniques: Amplification; Incubation; Selection
bioz_score: 94
title: Toward Understanding How Staphylococcal Protein A Inhibits IgG-Mediated Phagocytosis.
authors: Ana Rita Cruz; Arthur E H Bentlage; ...; Suzan H M Rooijakkers
journal: Journal of Immunology (Baltimore, Md. : 1950)
date: 2022-09-23
pmid: 36002230
doi: 10.4049/jimmunol.2200080
cited_by_count: 12
techniques: Produced
bioz_score: 94
title: Staphylococcal protein A inhibits IgG-mediated phagocytosis by blocking the interaction of IgGs with FcγRs and FcRn
authors: Cruz Ana Rita; Bentlage Arthur E. H.; ...; Rooijakkers Suzan H. M.
journal: bioRxiv
date: 2022-01-22
doi: 10.1101/2022.01.21.477287
cited_by_count: 0
techniques: Produced
bioz_score: 94
title: C-Reactive Protein Enhances IgG-Mediated Cellular Destruction Through IgG-Fc Receptors in vitro
authors: A. Robin Temming; Matthias Tammes Buirs; ...; Gestur Vidarsson
journal: Frontiers in Immunology
date: 2021-03-15
pmid: 33790888
doi: 10.3389/fimmu.2021.594773
pmcid: 8006934
cited_by_count: 11
bioz_score: 94
title: Cross-reactivity of mouse IgG subclasses to human Fc gamma receptors: Antibody deglycosylation only eliminates IgG2b binding.
authors: A Robin Temming; Arthur E H Bentlage; ...; Gestur Vidarsson
journal: Molecular immunology
date: 2020-11-30
pmid: 32947169
doi: 10.1016/j.molimm.2020.08.015
cited_by_count: 46
bioz_score: 94
title: FcRn is a CD32a coreceptor that determines susceptibility to IgG immune complex–driven autoimmunity
authors: Jonathan J. Hubbard; Michal Pyzik; ...; Richard S. Blumberg
journal: The Journal of Experimental Medicine
date: 2020-10-05
pmid: 32658257
doi: 10.1084/jem.20200359
pmcid: 7537387
cited_by_count: 45
techniques: Amplification; Binding Assay; Biomarker Discovery; Comparison; Concentration Assay; Control; Derivative Assay; Enzyme-linked Immunosorbent Assay; Expressing; Fluorescence; Histopathology; Hybridization; Immunoprecipitation; Incubation; Injection; Irradiation; Molecular Weight; Multiplex Assay; Mutagenesis; Plasmid Preparation; Stable Transfection; Staining; Titration; Transfection; Variant Assay; Western Blot
bioz_score: 94
title: FcγR Binding and ADCC Activity of Human IgG Allotypes
authors: Steven W. de Taeye; Arthur E. H. Bentlage; ...; Gestur Vidarsson
journal: Frontiers in Immunology
date: 2020-05-06
pmid: 32435243
doi: 10.3389/fimmu.2020.00740
pmcid: 7218058
cited_by_count: 124
techniques: Binding Assay; Comparison; Concentration Assay; Construct; Control; Expressing; Mutagenesis; Residue; SPR Assay; Sedimentation
bioz_score: 93
title: Enhanced Effector Functions Due to Antibody Defucosylation Depend on the Effector Cell Fcγ Receptor Profile.
authors: Christine W Bruggeman; Gillian Dekkers; ...; Taco W Kuijpers
journal: Journal of Immunology (Baltimore, Md. : 1950)
date: 2017-09-27
pmid: 28566370
doi: 10.4049/jimmunol.1700116
cited_by_count: 61
techniques: Clone Assay; Construct; Isolation; Produced; SPR Assay
bioz_score: 93
