Supplied as sterile 50 mM Hepes,150 mM NaCl, 10% Trehalose, Protease Inhibitor, pH 7.2. Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
Shipping
It is supplied and shipped as liquid with dry ice.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -70℃. Store it under sterile conditions at -70℃ or lower. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Activity
1.Immobilized Recombinant Human CCR7/CD197 Protein (VLP,Flag Tag) (Cat: 12112-H25H) at 5 μg/mL (100 μL/well) can bind JBH492-Derived Anti-CCR7 Antibody, Human IgG1, the EC 50 is 15-55 ng/mL(QC tested). 2.Immobilized Recombinant Human CCR7/CD197 Protein (VLP,Flag Tag) (Cat: 12112-H25H) at 5 μg/mL (100 μL/well) can bind CAP100-Derived Anti-CCR7 Antibody, Human IgG1, the EC 50 is 5-25 ng/mL(Routinely tested).
Protein Construction
A DNA sequence encoding the Human CCR7-VLP (Full Length) (P32248) (Met1-Leu344, thermostabilizing mutations) was fused with a flag tag at the C-terminus. This protein was prepared using our VLP platform. As an innovative technology platform, VLPs are generated through the self-assembly of viral proteins in HEK293 cells. This process efficiently integrates membrane proteins onto the VLP surface, preserving their native conformation and yielding soluble, high-concentration proteins that are ideally suited for immunization and antibody screening.
Expressed Host
HEK293 Cells
Species
Human
Molecule Mass
The recombinant Human CCR7 consists of 352 amino acids and predicts a molecular mass of 39.96 kDa.