| Catalog No. | Size | Price (USD) |
| E27-11G-10 | 10 µg | $515.43 |
| E27-11G-50 | 50 µg | $1,729.93 |
| E27-11G-500 | 500 µg | $3,907.16 |
General Information
| Formulation | Supplied as sterile 50 mM Tris-HCl, pH 7.5, 50-300mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25-1.0mM DTT, 0-0.1mM PMSF, 10-25% glycerol Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| Shipping | Enzymes are highly recommended to be shipped at frozen temperature with dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products. |
| Stability & Storage | Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. |
| Purity | For specific information on a given lot, see related technical data sheet. |
| Activity | For specific information on a given lot, see related technical data sheet. |
| Protein Construction | Recombinant human HER2 (676-end) was expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag. |
| Expressed Host | Baculovirus-Insect Cells |
| Species | Human |
| Molecule Mass | ~116 kDa |
| Technical Data Sheet by Lot # | A4603-2 A4651-2 J3861-11 J3861-11B N5368-6 N5385-10 Y4317-12 |
Synonyms: c-ERB-2 Protein, Human; c-ERB2 Protein, Human; CD340 Protein, Human; HER-2 Protein, Human; HER-2/neu Protein, Human; HER2 Protein, Human; MLN 19 Protein, Human; MLN-19 Protein, Human; MLN19 Protein, Human; NEU Protein, Human; NGL Protein, Human; p185(erbB2) Protein, Human; TKR1 Protein, Human; VSCN2 Protein, Human
Image / Experimental Information
| Image 1 Description | Determination of ATP Km for recombinant protein kinases HER2 (Cat. No.: E27-11G) using ADP-GloTM Assay. With saturating substrate concentration, the relative luminescence values were plotted against various ATP concentrations. The Michaelis-Menten equation was then used to generate the Km values. (Validation Experiment) |
| Image 1 Alt Text | Human Her2/ERBB2 Protein 46438 |
| Image 1 URL | Source image |
Background Information
| Full Name | erb-b2 receptor tyrosine kinase 2 |
| Description | Human epidermal growth factor receptor 2 (HER2), also known as ErbB2, NEU, and CD340, is a type I membrane glycoprotein and belongs to the epidermal growth factor (EGF) receptor family. HER2 protein cannot bind growth factors due to the lacking of ligand binding domain of its own and autoinhibited constitutively. However, HER2 forms a heterodimer with other ligand-bound EGF receptor family members, therefore stabilizes ligand binding and enhances kinase-mediated activation of downstream molecules. HER2 plays a key role in development, cell proliferation and differentiation. HER2 gene has been reported to associate with malignancy and a poor prognosis in numerous carcinomas, including breast, prostate, ovarian, lung cancers and so on. |
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| References |
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title: Synergistic Role of MoO₃–CTAB Nanostructures in Boosting Electrochemical Immunosensing of HER2
journal: Electrochimica Acta
date: 2025-12-01
doi: 10.1016/j.electacta.2025.148092
cited_by_count: 0
techniques: Recombinant
bioz_score: 93
title: Photoinduced Actin Aggregation Involves Cell Death: A Mechanism of Cancer Cell Cytotoxicity after Near-Infrared Photoimmunotherapy
authors: Kazuhide Sato; Tomoko Okada; ...; Toshihiko Ogura
journal: ACS Nano
date: 2025-02-18
pmid: 39964399
pmcid: 11887486
cited_by_count: 3
techniques: Comparison; Concentration Assay; Control; Ex Vivo; Expressing; Flow Cytometry; Fluorescence; Imaging; Immunoprecipitation; In Vitro; In Vivo; Incubation; Inhibition; Irradiation; Magnetic Beads; Membrane; Microscopy; Recombinant; SDS Page; Staining; Suspension; Western Blot
bioz_score: 93
title: Formation of mono- and dual-labelled antibody fragment conjugates via reversible site-selective disulfide modification and proximity induced lysine reactivity
authors: Ioanna A. Thanasi; Nathalie Bouloc; ...; Vijay Chudasama
journal: Chemical Science
date: 2025-01-06
doi: 10.1039/d4sc06500j
pmcid: 11726237
cited_by_count: 0
techniques: Enzyme-linked Immunosorbent Assay; Glycoproteomics; Incubation; Modification; Residue; Sequencing; Software
bioz_score: 93
title: Unveiling the intra-tumor fate of trastuzumab deruxtecan in a xenograft model to support its mechanism of action.
authors: Yoko Nagai; Masataka Oitate; ...; Nobuaki Watanabe
journal: Drug metabolism and pharmacokinetics
date: 2024-06-22
pmid: 38643548
doi: 10.1016/j.dmpk.2024.101001
cited_by_count: 2
techniques: Antibody Labeling; Fluorescence; In Vitro; In Vivo Imaging; Labeling; Recombinant; SPR Assay; Software
bioz_score: 93
title: Acquired secondary HER2 mutations enhance HER2/MAPK signaling and promote resistance to HER2 kinase inhibition in breast cancer
authors: Arnaldo Marín; Abdullah Al Mamun; ...; Ariella B. Hanker
journal: Cancer Research
date: 2024-03-15
pmid: 37404061
doi: 10.1158/0008-5472.CAN-22-3617
pmcid: 10530374
cited_by_count: 21
techniques: Activation Assay; Binding Assay; Cell Analysis; Construct; Expressing; Fluorescence; Glo Assay; Inhibition; Mutagenesis; Stable Transfection; Staining; Western Blot
bioz_score: 93
title: Antibody-mediated delivery of CRISPR-Cas9 ribonucleoproteins in human cells.
authors: Stephanie Ubiparipovic; Daniel Christ; Romain Rouet
journal: Protein engineering, design & selection : PEDS
date: 2022-12-07
pmid: 36336952
doi: 10.1093/protein/gzac011
cited_by_count: 3
techniques: Recombinant
bioz_score: 93
title: HER3 ligands and uses thereof
authors: Christopher Novotny; Kevan Shokat; Natalia Jura
date: 2021-09-21
cited_by_count: 0
techniques: Fluorescence; In Vitro; Incubation; Kinase Assay; Polymerase Chain Reaction; Reverse Transcription Polymerase Chain Reaction
bioz_score: 93
title: Decorating sdAbs with Chelators: Effect of Conjugation on Biodistribution and Functionality
authors: Henri Baudhuin; Janik Puttemans; ...; Gerald Reischl
journal: Pharmaceuticals
date: 2021-04-25
pmid: 33923088
doi: 10.3390/ph14050407
pmcid: 8146233
cited_by_count: 5
techniques: Binding Assay; Ex Vivo; Expressing; Imaging; Purification; Recombinant; SPR Assay; Single Photon Emission Computed Tomography
bioz_score: 93
title: Anti-cancer fusion polypeptide
authors: Marlon Hinner; Christine Rothe; ...; Thomas Jean Jaquin
date: 2020-12-15
cited_by_count: 0
techniques: Enzyme-linked Immunosorbent Assay; Incubation; Recombinant
bioz_score: 93
title: A HER2 DNA Vaccine with Evolution-Selected Amino Acid Substitutions Reveals a Fundamental Principle for Cancer Vaccine Formulation in HER2 Transgenic Mice
authors: Richard F. Jones; Joyce D. Reyes; ...; Wei-Zen Wei
journal: Cancer Immunology, Immunotherapy : CII
date: 2020-07-01
pmid: 31177328
doi: 10.1007/s00262-019-02333-9
pmcid: 6586491
cited_by_count: 2
techniques: Alamar Blue Assay; Binding Assay; Clone Assay; Construct; Enzyme-linked Immunospot; Expressing; Flow Cytometry; Fluorescence; In Vitro; In Vivo; Incubation; Inhibition; Injection; Labeling; Negative Control; Peptide Microarray; Plasmid Preparation; Positive Control; Recombinant; Transfection
bioz_score: 94
title: High-Affinity Antibody Detection with a Bivalent Circularized Peptide Containing Antibody-Binding Domains.
authors: Fangyu Zhou; Andrew Kroetsch; ...; Sheldon Park
journal: Biotechnology journal
date: 2019-10-24
pmid: 30810268
doi: 10.1002/biot.201800647
cited_by_count: 4
techniques: Avidin-Biotin Assay; Recombinant
bioz_score: 93
title: Overcoming resistance to HER2 inhibitors through state-specific kinase binding.
authors: Chris J Novotny; Sirkku Pollari; ...; Kevan M Shokat
journal: Nature Chemical Biology
date: 2017-05-15
pmid: 27595329
doi: 10.1038/nchembio.2171
cited_by_count: 22
techniques: In Vitro; Incubation; Kinase Assay; Proliferation Assay
bioz_score: 94
title: Surrogate in vitro activation of innate immunity synergizes with interleukin-7 to unleash rapid antigen-driven outgrowth of CD4+ and CD8+ human peripheral blood T-cells naturally recognizing MUC1, HER2/neu and other tumor-associated antigens
authors: Latha B. Pathangey; Dustin B. McCurry; ...; Peter A. Cohen
journal: Oncotarget
date: 2017-02-14
pmid: 27974697
doi: 10.18632/oncotarget.13911
pmcid: 5355224
cited_by_count: 13
techniques: Binding Assay; Cell Culture; Concentration Assay; Derivative Assay; Enzyme-linked Immunosorbent Assay; Expressing; Immunocytochemistry; Recombinant; Software; Two Tailed Test
bioz_score: 93
title: Purification and immunodetection of the complete recombinant HER-2[neu] receptor produced in yeast.
authors: Alexandre Chamas; Martin Giersberg; ...; Gotthard Kunze
journal: Protein expression and purification
date: 2016-09-30
pmid: 25450238
doi: 10.1016/j.pep.2014.10.004
cited_by_count: 5
techniques: Functional Assay; Kinase Assay
bioz_score: 93
MDSD
