| Catalog No. | Size | Price (USD) |
| 10369-HNAC-1 | 1 mg | $4,321.87 |
| 10369-HNAC-5 | 5 µg | $85.90 |
| 10369-HNAC-20 | 20 µg | $293.26 |
| 10369-HNAC-100 | 100 µg | $663.54 |
| 10369-HNAC-500 | 500 µg | $3,699.81 |
General Information
| Formulation | Lyophilized from sterile PBS, 10% Trehalose, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| Endotoxin | <10 EU per mg protein. |
| Shipping | In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
| Stability & Storage | Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Reconstitution | To find reconstitution info, click on 'COA' at the top of the page, enter the lot number and product size for the lot-specific COA. |
| Purity | ≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by HPLC. ≥ 95% as determined by SEC-MALS(Routinely tested). |
| Activity | 1.Measured in a cell proliferation assay using TF1 human erythroleukemic cells (Kitamura, T. et al. 1989, J. Cell Physiol. 140:323.). The ED 50 for this effect is typically 0.5-3 ng/mL. 2.Loaded Recombinant Human IL-4Rα & IL-13Rα1 Heterodimer Protein, hFc Tag (Cat. No. CT117-H02H) on proA Biosensor, can bind Recombinant Human IL-13 Protein (Cat. No. 10369-HNAC) with an affinity constant of 1.690 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested). 3.Loaded Recombinant Human IL13RA1 Protein (ECD, Fc Tag) (Cat. No. 10943-H02H) on ProA Biosensor, can bind Recombinant Human IL13 Protein (Cat. No. 10369-HNAC) with an affinity constant of 17.6 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested). 4.Recombinant Human IL13RA1 Protein (ECD, Fc Tag) (Cat: 10943-H02H) captured on Protein A chip, can bind Recombinant Human IL13 Protein (Cat: 10369-HNAC) with an affinity constant of 9.560 nM as determined in an SPR assay (Biacore T200) (Routinely tested). 5.Recombinant Human IL13RA1 Protein (ECD, His & AVI Tag), Biotinylated (Cat: 10943-H49H-B) captured on NTA chip, can bind Recombinant Human IL13 Protein (Cat: 10369-HNAC) with an affinity constant of 3.366 nM as determined in an SPR assay (Biacore T200) (Routinely tested). |
| Protein Construction | A DNA sequence encoding the human IL13 (AAK53823.1) (Met 1-Asn 132) was expressed and purified. |
| Accession# | AAK53823.1 |
| Expressed Host | CHO Stable Cells |
| Species | Human |
| Predicted N Terminal | Gly 21 |
| Molecule Mass | The recombinant human IL13 consists of 112 amino acids and migrates as an approximately 12.35 kDa band in SDS-PAGE under reducing conditions as predicted. |
Synonyms: ALRH Protein, Human; BHR1 Protein, Human; IL-13 Protein, Human; MGC116786 Protein, Human; MGC116788 Protein, Human; MGC116789 Protein, Human; NC30 Protein, Human; P600 Protein, Human
Image / Experimental Information
| Image 1 Alt Text | Human IL13/IL-13/ALRH Protein 8728 |
| Image 1 URL | Source image |
| Image 2 Alt Text | Human IL-13 Protein 32981 |
| Image 2 URL | Source image |
| Image 3 Alt Text | Human IL-13 Protein 42681 |
| Image 3 URL | Source image |
| Image 4 Alt Text | Human IL-13 Protein 42682 |
| Image 4 URL | Source image |
| Image 5 Alt Text | Human IL-13 Protein 43421 |
| Image 5 URL | Source image |
Background Information
| Full Name | interleukin 13 |
| Description | Interleukin 13 (IL-13) is a single-chain glycosylated polypeptide, which belongs to the IL-13/IL-4 family. IL-13 protein is secreted by many cell types, but especially by T helper type 2 (Th2) cells. IL-13 exerts its effects through a multi-subunit receptor comprising the alpha chain of the IL-4 receptor (IL-4Rα) and at least one of two known IL-13-specific binding chains (IL-13 Rα1 and IL-13 Rα2). As a cytokine, IL-13 protein is critical in regulating inflammatory, immune responses, and diseases. Also, it inhibits the production of pro-inflammatory cytokines and chemokines, and thus down-regulates macrophage activity. IL-13 protein and antibody are more importantly implicated as a central mediator of immunoregulatory processes in various cell types. |
| Research Areas |
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| Tags |
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| References |
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title: Exosomal NGFR derived from gastric cancer drives PANoptosis of M2 macrophages by activating RIPK1 via phosphorylation
authors: Huiru Yang; Zhekai Zhang; ...; Haifeng Jin
journal: Journal of Radiation Research and Applied Sciences
date: 2026-04-08
doi: 10.1016/j.jrras.2026.102348
cited_by_count: 0
techniques: Cell Culture; Incubation; Saline
bioz_score: 95
title: Colorectal cancer-derived osteopontin rewires macrophages into a pro-metastatic M2 state via the PI3K/AKT/CSF1-CSF1R axis
authors: Xiaoxia Liang; Fei Qin; ...; Dianke Chen
journal: Cell Death Discovery
date: 2026-02-05
pmid: 41639044
doi: 10.1038/s41420-026-02945-y
cited_by_count: 0
techniques: Incubation
bioz_score: 95
title: Polypeptides comprising immunoglobulin single variable domains targeting il-13 and OX40L
authors: Heidi Rommelaere; Ann Brigé; ...; Bernd Weigle
date: 2025-12-30
cited_by_count: 0
techniques: Concentration Assay; Enzyme-linked Immunosorbent Assay; Expressing; In Vitro; Incubation; Inhibition
bioz_score: 95
title: Methods of treatment utilizing polypeptides comprising immunoglobulin single variable domains targeting IL-13 and TSLP
authors: Heidi Rommelaere; Ann Brigé; ...; Klaus Erb
date: 2025-12-09
cited_by_count: 0
techniques: Construct; Derivative Assay; Incubation; Recombinant
bioz_score: 95
title: Nucleic acids encoding polypeptides comprising immunoglobulin single variable domains targeting IL-13 and TSLP
authors: Heidi Rommelaere; Ann Brigé; ...; Klaus Erb
date: 2025-08-12
cited_by_count: 0
techniques: Construct; Derivative Assay; Recombinant
bioz_score: 95
title: Interleukin-4 receptor antibody and application thereof
authors: Deyong Song; Chuangchuang Dong; Jing Han
date: 2025-06-24
cited_by_count: 0
techniques: Blocking Assay; Comparison; Concentration Assay; Enzyme-linked Immunosorbent Assay; Incubation; Protein Binding; Sequencing
bioz_score: 95
title: Metformin Inhibited GSDME to Suppress M2 Macrophage Pyroptosis and Maintain M2 Phenotype to Mitigate Cisplatin-Induced Intestinal Inflammation
authors: Ke Jiang; Qi He; ...; Amirata Saei Dibavar
journal: Biomedicines
date: 2024-11-04
pmid: 39595093
doi: 10.3390/biomedicines12112526
pmcid: 11592070
cited_by_count: 0
techniques: Cell Culture; Concentration Assay; Incubation
bioz_score: 93
title: Crosstalk between GBP2 and M2 macrophage promotes the ccRCC progression
authors: Wei Zheng; Shujiang Ye; ...; Guangyuan Li
journal: Cancer Science
date: 2024-09-02
pmid: 39222374
doi: 10.1111/cas.16287
pmcid: 11531969
cited_by_count: 7
techniques: Cell Culture; Co-Culture Assay; Expressing; Migration; Real-time Polymerase Chain Reaction; Western Blot; Wound Healing Assay
bioz_score: 95
title: Methods of treating asthma with polypeptides targeting IL-13 and TSLP
authors: Heidi Rommelaere; Ann Brigé; ...; Klaus Erb
date: 2023-12-12
cited_by_count: 0
techniques: Construct; Derivative Assay; Recombinant
bioz_score: 95
title: Autoimmune suppressor and application thereof
authors: Bo Chen; Gang Xu; Juntao Yu
date: 2023-08-15
cited_by_count: 0
techniques: Binding Assay; Concentration Assay; Inhibition; Software; Transferring
bioz_score: 94
title: Proteomics of immune cells from liver tumors reveals immunotherapy targets
authors: Fernando P. Canale; Julia Neumann; ...; Roger Geiger
journal: Cell Genomics
date: 2023-05-30
pmcid: 10300607
cited_by_count: 1
techniques: Cell Isolation; DNA Extraction; Enzyme-linked Immunosorbent Assay; Expressing; Flow Cytometry; In Vitro; Isolation; Knock-Out; Modification; Mouse Assay; Recombinant; Sequencing; Software; Transduction; Transfection; Two Tailed Test
bioz_score: 95
title: IL-13RA2 downregulation in fibroblasts promotes keloid fibrosis via JAK/STAT6 activation
authors: Hua Chao; Lisheng Zheng; ...; Qing Tang
journal: JCI insight
date: 2023-03-22
pmid: 36757802
pmcid: 10070111
cited_by_count: 25
techniques: Staining; TUNEL Assay
bioz_score: 93
title: Identification of FCN1 as a novel macrophage infiltration-associated biomarker for diagnosis of pediatric inflammatory bowel diseases.
authors: Xixi Chen; Yuanqi Gao; ...; Pu Li
journal: Journal of Translational Medicine
date: 2023-03-21
pmid: 36932401
doi: 10.1186/s12967-023-04038-1
cited_by_count: 25
techniques: Cell Culture; Control; Incubation; Over Expression; Transfection
bioz_score: 95
title: mTOR inhibitor, gemcitabine and PD-L1 antibody blockade combination therapy suppresses pancreatic cancer progression via metabolic reprogramming and immune microenvironment remodeling in Trp53 flox/+ LSL-Kras G12D/+ Pdx-1-Cre murine models.
authors: Jiangdong Qiu; Mengyu Feng; ...; Taiping Zhang
journal: Cancer letters
date: 2023-01-11
pmid: 36442772
doi: 10.1016/j.canlet.2022.216020
cited_by_count: 25
techniques: Cell Culture; Incubation
bioz_score: 95
title: Lycium barbarum polysaccharide alleviates dextran sodium sulfate-induced inflammatory bowel disease by regulating M1/M2 macrophage polarization via the STAT1 and STAT6 pathways.
authors: Juan Wang; Huiying Gao; ...; Haibin Xia
journal: Frontiers in Pharmacology
date: 2022-09-14
pmid: 37144216
doi: 10.3389/fphar.2023.1044576
cited_by_count: 16
techniques: Cell Culture; Generated; Protein-Protein interactions
bioz_score: 95
title: Silence of a dependence receptor CSF1R in colorectal cancer cells activates tumor-associated macrophages
authors: Mingxuan Zhu; Liangliang Bai; ...; Yanxin Luo
journal: Journal for Immunotherapy of Cancer
date: 2022-01-01
pmid: 36600555
doi: 10.1136/jitc-2022-005610
pmcid: 9730427
cited_by_count: 49
techniques: Caspase-Glo Assay; Cell Counting; Clinical Proteomics; Control; DNA Methylation Assay; Enzyme-linked Immunosorbent Assay; Membrane; Methylation; Plasmid Preparation; Polymerase Chain Reaction; Purification; Recombinant; SYBR Green Assay; Sequencing
bioz_score: 95
title: Development of a potent high-affinity human therapeutic antibody via novel application of recombination signal sequence–based affinity maturation
authors: Agnieszka Kielczewska; Igor D'Angelo; ...; Ian N. Foltz
journal: The Journal of Biological Chemistry
date: 2021-12-29
pmid: 34973336
doi: 10.1016/j.jbc.2021.101533
pmcid: 8808179
cited_by_count: 5
techniques: Bioassay; Cell Culture; Concentration Assay; Control; Inhibition; Recombinant; Suspension
bioz_score: 95
title: Polypeptides comprising immunoglobulin single variable domains targeting IL-13 and TSLP
authors: Heidi Rommelaere; Ann Brigé; ...; Klaus Erb
date: 2021-12-28
cited_by_count: 0
techniques: Construct; Derivative Assay; Recombinant
bioz_score: 95
title: Development of Neutralizing Multimeric Nanobody Constructs Directed against IL-13: From Immunization to Lead Optimization.
authors: Philippe J-L Y Gevenois; Pieter De Pauw; ...; Nathalie Wauthoz
journal: Journal of Immunology (Baltimore, Md. : 1950)
date: 2021-11-26
pmid: 34645688
doi: 10.4049/jimmunol.2100250
cited_by_count: 7
techniques: Amplification; Clone Assay; Concentration Assay; Enzyme-linked Immunosorbent Assay; In Vitro; Plasmid Preparation; Transformation Assay; Variant Assay
bioz_score: 95
title: IL-13 antibody and preparation method and use thereof
authors: Yizhen Yang; Shiyong Gong; ...; Lile Liu
date: 2021-10-05
cited_by_count: 0
techniques: Activity Assay; Concentration Assay; Enzyme-linked Immunosorbent Assay; Positive Control
bioz_score: 95
