| Catalog No. | Size | Price (USD) |
| J01-11G-10 | 10 µg | $559.86 |
| J01-11G-50 | 50 µg | $1,959.51 |
| J01-11G-500 | 500 µg | $4,129.33 |
General Information
| Formulation | Supplied as sterile 50 mM Tris-HCl, pH 7.5, 50-300mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25-1.0mM DTT, 0-0.1mM PMSF, 10-25% glycerol Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| Shipping | Enzymes are highly recommended to be shipped at frozen temperature with dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products. |
| Stability & Storage | Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. |
| Purity | For specific information on a given lot, see related technical data sheet. |
| Activity | For specific information on a given lot, see related technical data sheet. |
| Protein Construction | Recombinant human JAK1 (438-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. |
| Expressed Host | Baculovirus-Insect Cells |
| Species | Human |
| Molecule Mass | ~108 kDa |
| Technical Data Sheet by Lot # | A4609-12 K4488-7 L5120-11 L5153-19 T4143-6 T4157-2 Z4981-5 Z4981-6 |
Synonyms: AIIDE Protein, Human; JAK1A Protein, Human; JAK1B Protein, Human; JTK3 Protein, Human
Image / Experimental Information
| Image 1 Description | Determination of ATP Km for recombinant protein kinases JAK1 (Cat. No.: J01-11G) using ADP-GloTM Assay. With saturating substrate concentration, the relative luminescence values were plotted against various ATP concentrations. The Michaelis-Menten equation was then used to generate the Km values. (Validation Experiment) |
| Image 1 Alt Text | Human JAK1 Protein 46457 |
| Image 1 URL | Source image |
| Image 2 Description | Determination of ATP Km for recombinant protein kinases JAK1 (Cat. No.: J01-11G) using ADP-GloTM Assay. With saturating substrate concentration, the relative luminescence values were plotted against various ATP concentrations. The Michaelis-Menten equation was then used to generate the Km values. (Validation Experiment) |
| Image 2 Alt Text | Human JAK1 Protein 49487 |
| Image 2 URL | Source image |
Background Information
| Full Name | Janus kinase 1 |
| Description | JAK1 is a member of protein-tyrosine kinases (PTK) characterized by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. JAK1 bears all the hallmarks of a protein kinase, although its structure differs significantly from that of the PTK and threonine/serine kinase family members. JAK1 is a large, widely expressed membrane-associated phosphoprotein that is involved in the interferon-alpha/beta and -gamma signal transduction pathways. JAK1 plays an essential and nonredundant role in promoting biologic responses induced by a select subset of cytokine receptors, including those in which JAK utilization is thought to be nonspecific. |
| Research Areas |
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| Related Pathways |
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title: Discovery of Natural Compound α‐Hederin via Large‐Scale Screening as a Targeted JAK/STAT3 Inhibitor for Ovarian Cancer Therapy
authors: Jiayu Wang; Pengzhan He; ...; Zhinang Yin
journal: Advanced Science
date: 2025-07-16
pmid: 40667652
doi: 10.1002/advs.202417278
cited_by_count: 4
techniques: Activation Assay; Activity Assay; Binding Assay; Biomarker Discovery; Competitive Binding Assay; Expressing; Fluorescence; Generated; In Vitro; Incubation; Kinase Assay; Microscale Thermophoresis; Migration; Phospho-proteomics; Protease Inhibitor; Pull Down Assay; Purification; Quantitative RT-PCR; Recombinant; Software; Stability Assay; Staining; Translocation Assay; Two Tailed Test; Western Blot
bioz_score: 93
title: Epinephrine inhibits PI3Kα via the Hippo kinases
authors: Ting-Yu Lin; Shakti Ramsamooj; ...; Marcus D. Goncalves
journal: Cell Reports
date: 2024-01-10
pmid: 38060450
doi: 10.1016/j.celrep.2023.113535
pmcid: 10809223
cited_by_count: 1
techniques: Double Knockout; Fluorescence; Isolation; Mutagenesis; Plasmid Preparation; Recombinant; Software
bioz_score: 93
title: Selective inhibitors of JAK1 targeting an isoform-restricted allosteric cysteine.
authors: Madeline E Kavanagh; Benjamin D Horning; ...; Benjamin F Cravatt
journal: Nature Chemical Biology
date: 2022-11-25
pmid: 36097295
doi: 10.1038/s41589-022-01098-0
cited_by_count: 37
techniques: Activity Assay; Purification; Recombinant
bioz_score: 93
title: Selective inhibitors of JAK1 targeting a subtype-restricted allosteric cysteine
authors: Kavanagh Madeline E.; Horning Benjamin D.; ...; Cravatt Benjamin F.
journal: bioRxiv
date: 2022-02-01
doi: 10.1101/2022.01.31.478302
cited_by_count: 0
techniques: Activity Assay; Binding Assay; Concentration Assay; Construct; Derivative Assay; Ex Vivo; Expressing; Generated; In Situ; In Vitro; Inhibition; Labeling; Mutagenesis; Purification; Recombinant; Scaffolding; Sequencing; Transfection; Transformation Assay; Variant Assay; Western Blot
bioz_score: 93
MDSD
