| Catalog No. | Size | Price (USD) |
| 11848-HNAE2-20 | 20 µg | $115.53 |
| 11848-HNAE2-50 | 50 µg | $159.96 |
| 11848-HNAE2-500 | 500 µg | $737.59 |
General Information
| Formulation | Lyophilized from sterile 20mM HAc-NaAc+0.004% Tween-80 pH 4.0 Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| Endotoxin | < 5 EU per mg of the protein. |
| Shipping | In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
| Stability & Storage | Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Reconstitution | To find reconstitution info, click on 'COA' at the top of the page, enter the lot number and product size for the lot-specific COA. |
| Purity | ≥ 95 % as determined by SDS-PAGE.≥ 95 % as determined by SEC-HPLC. |
| Activity | 1.Measured in a cell proliferation assay using CTLL2. The ED 50 for this effect is typically 1-8 ng/mL. 2.Loaded Recombinant Rhesus IL-2R beta/CD122 Protein, His & AVI Tag, Biotinylated (Cat. No. 90328-K49H-B) on SA Biosensor, can bind Recombinant Human IL-2 Protein, no Tag (Cat. No. 11848-HNAE2) with an affinity constant of 0.508 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested). 3.Loaded Recombinant Human IL-2R beta / CD122 Protein, His Tag (Cat. No. 10696-H08H) on Ni-NTA Biosensor, can bind Recombinant Human IL-2 Protein (Cat. No. 11848-HNAE2) with an affinity constant of 0.313 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested). 4.Loaded Recombinant Human IL-2R beta/CD122 Protein, His Tag, Biotinylated, (Cat. No. 10696-H27H-B) on SA Biosensor, can bind Recombinant Human IL-2 Protein (Cat. No. 11848-HNAE2) with an affinity constant of 0.234 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested). 5.Recombinant Human IL-2R beta/CD122 Protein, mFc Tag (Cat. No. 10696-H05H) captured on CM5 chip via anti-mouse antibodies surface can bind Recombinant Human IL-2 Protein (Cat. No. 11848-HNAE2) with an affinity constant of 0.460 µM as determined in an SPR assay (Biacore T200) (Routinely tested). 6.Loaded Biotinylated Recombinant Human IL-2Rβ & IL-2Rγ Protein, Flag & AVI & His Tag (Cat. No. CT108-H2549H1-B) on SA Biosensor, can bind Recombinant Human IL-2 Protein (Cat. No. 11848-HNAE2) with an affinity constant of 2.190 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested). |
| Protein Construction | A DNA sequence encoding the mature form of human IL2 (P60568-1) (Ala21-Thr153) was expressed and purified with an initial Met. |
| Accession# | P60568-1 |
| Expressed Host | E. coli |
| Species | Human |
| Predicted N Terminal | Met |
| Molecule Mass | The recombinant human IL-2 consists of 134 amino acids and predicts a molecular mass of 15.55 kDa. |
Synonyms: IL-2 Protein, Human; lymphokine Protein, Human; TCGF Protein, Human
Image / Experimental Information
| Image 1 Alt Text | Human IL2 Protein 35240 |
| Image 1 URL | Source image |
| Image 2 Alt Text | Human IL2 Protein 35247 |
| Image 2 URL | Source image |
| Image 3 Alt Text | Human IL2 Protein 35250 |
| Image 3 URL | Source image |
| Image 4 Alt Text | Human IL-2 Protein 40607 |
| Image 4 URL | Source image |
| Image 5 Alt Text | Human IL-2 Protein 41004 |
| Image 5 URL | Source image |
Background Information
| Full Name | interleukin 2 |
| Description | Interleukin-2, also known as a T-cell growth factor, TCGF, and IL2, is a secreted protein that belongs to the IL-2 family. Interleukin-2 / IL-2 was the first interleukin molecule to be discovered. Interleukin-2 / IL-2 molecule was first purified to homogeneity by immunoaffinity chromatography by Kendall Smith and his team at Dartmouth Medical School. Interleukin-2 / IL-2 was also the first cytokine shown to mediate its effects via a specific IL-2 receptor, and it was also the first interleukin to be cloned and expressed from a complementary DNA (cDNA) library. Interleukin-2 / IL-2 was designated number 2 because Smith's data at the time indicated that IL-1, produced by macrophages, facilitates IL-2 production by T lymphocytes (T cells). Interleukin-2 / IL-2 is produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Interleukin-2 / IL-2 is normally produced by the body during an immune response. When environmental substances (molecules or microbes) gain access to the body, these substances (termed antigens) are recognized as foreign by antigen receptors that are expressed on the surface of lymphocytes. Antigen binding to the T cell receptor (TCR) stimulates the secretion of Interleukin-2 / IL-2 and the expression of IL-2 receptors IL-2R. The IL-2 / IL-2R interaction then stimulates the growth, differentiation, and survival of antigen-selected cytotoxic T cells via the activation of the expression of specific genes. Interleukin-2 / IL-2 can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. The World Reference Standard for Interleukin-2 / IL-2 is produced by the National Institute of Biological Standards and Control in the UK. A recombinant form of Interleukin-2 / IL-2 for clinical use is manufactured by Chiron Corporation. It has been approved by the Food and Drug Administration (FDA) for the treatment of cancers (malignant melanoma, renal cell cancer), and is in clinical trials for the treatment of chronic viral infections, and as a booster (adjuvant) for vaccines. The use of Interleukin-2 / IL-2 in HIV therapy is ineffective. |
| Research Areas |
|
| Related Pathways |
|
| Tags |
|
| References |
|
title: Low-intensity pulsed ultrasound-mediated nose-to-brain co-delivery of β-blockers and aPD-L1 enhances glioblastoma immunotherapy
authors: Lei Dong; Zhengcheng Yun; ...; Jinbing Xie
journal: Nature Communications
date: 2026-07-30
pmid: 42669690
doi: 10.1038/s41467-026-76103-4
cited_by_count: 0
techniques: Cell Culture; Isolation; Recombinant
bioz_score: 94
title: TIMP1 and PKM drive immunosuppression and metabolic remodeling to promote colorectal cancer progression through integrated multi-omics analysis.
authors: Yukai Wang; Wei Zhang; ...; Xiaolong Tang
journal: International Journal of Biological Macromolecules
date: 2026-04-09
pmid: 41966374
doi: 10.1016/j.ijbiomac.2026.151944
cited_by_count: 0
techniques: Activation Assay; Cell Culture; Transduction
bioz_score: 94
title: Identification and characterization of bone marrow plasma cells producing IFN-γ-neutralizing autoantibodies in adult-onset immunodeficiency.
authors: Han Wang; Qianqian Yang; ...; Linqi Zhang
journal: Cell Reports Medicine
date: 2026-03-09
pmid: 41806838
doi: 10.1016/j.xcrm.2026.102657
cited_by_count: 0
techniques: Amplification; Binding Assay; Centrifugation; Enzyme-linked Immunosorbent Assay; Flow Cytometry; Irradiation; Lysis; Recombinant; Reverse Transcription
bioz_score: 94
title: Receptor-based bioassay for functional IL-2 quantification
authors: Chuanwei Lu; Shuangshuang Xu; ...; Grace Gong
journal: Journal of Pharmaceutical and Biomedical Analysis
date: 2025-04-01
doi: 10.1016/j.jpba.2025.116913
cited_by_count: 0
techniques: Bioassay; Functional Assay; Recombinant
bioz_score: 94
title: Investigating the therapeutic potential of Elemene emulsion injection as an adjuvant for chimeric antigen receptor T cell therapy: Transcriptome analysis and experimental validation
authors: Xiuying Liu; Jingjing Zhu; ...; Jianxun Wang
journal: European Journal of Integrative Medicine
date: 2025-01-01
doi: 10.1016/j.eujim.2024.102425
cited_by_count: 0
techniques: Activation Assay; Cell Culture; Flow Cytometry; Transduction
bioz_score: 94
title: Nanofilament immunotherapy induces potent antitumor vaccine responses
authors: Kevin Neil; Samuel Génier; ...; Jean-François Millau
journal: Journal for Immunotherapy of Cancer
date: 2025-01-01
pmid: 40889802
doi: 10.1136/jitc-2024-011331
cited_by_count: 1
techniques: Activity Assay; Generated; Incubation; Phage Preparation; Recombinant; Reporter Assay; Saline; Sterility
bioz_score: 94
title: Afucosylated anti-EBOV antibody MIL77-3 engages sGP to elicit NK cytotoxicity
authors: Yuting Zhang; Min Zhang; ...; Shan-Lu Liu
journal: Journal of virology
date: 2024-09-01
pmid: 39162435
doi: 10.1128/jvi.00685-24
pmcid: 11406966
cited_by_count: 0
techniques: Cell Culture; Cell Isolation; Centrifugation; Selection
bioz_score: 94
title: NAV-003, A Bispecific Antibody Targeting A Unique Mesothelin Epitope and CD3ε With Improved Cytotoxicity Against Humoral Immunosuppressed Tumors
authors: Luigi Grasso; Qun Jiang; ...; J. Bradford Kline
journal: European Journal of Immunology
date: 2024-08-01
pmid: 37146241
doi: 10.1002/eji.202250309
pmcid: 10524251
cited_by_count: 4
techniques: ADCC Assay; Activation Assay; Cell Culture; Comparison; Incubation; Luciferase; Produced; Recombinant; Two Tailed Test
bioz_score: 94
title: Heterologous survey of 130 DNA transposons in human cells highlights their functional divergence and expands the genome engineering toolbox.
authors: Tongtong Zhang; Shengjun Tan; ...; Yong E Zhang
journal: Cell
date: 2024-07-12
pmid: 38843831
doi: 10.1016/j.cell.2024.05.007
cited_by_count: 10
techniques: Conjugation Assay; Construct; Lysis; Recombinant; Staining; Virus
bioz_score: 94
title: Intratumoral delivery of a Tim-3 antibody-encoding oncolytic adenovirus engages an effective antitumor immune response in liver cancer
authors: qiang li; huii Zhang; ...; Yiqiang Wang
journal: Europe PMC
date: 2023-08-11
doi: 10.21203/rs.3.rs-3239891
cited_by_count: 0
techniques: Cell Culture; Centrifugation; Incubation; Isolation
bioz_score: 93
