| Catalog No. | Size | Price (USD) |
| M02-10G-10 | 10 µg | $515.43 |
| M02-10G-50 | 50 µg | $1,729.93 |
| M02-10G-500 | 500 µg | $3,907.16 |
General Information
| Formulation | Supplied as sterile 50 mM Tris-HCl, pH 7.5, 50-300mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25-1.0mM DTT, 0-0.1mM PMSF, 10-25% glycerol Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| Shipping | Enzymes are highly recommended to be shipped at frozen temperature with dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products. |
| Stability & Storage | Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. |
| Purity | For specific information on a given lot, see related technical data sheet. |
| Activity | For specific information on a given lot, see related technical data sheet. |
| Protein Construction | Recombinant full length human MEK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. |
| Expressed Host | Baculovirus-Insect Cells |
| Species | Human |
| Molecule Mass | ~72 kDa |
| Technical Data Sheet by Lot # | C2043-6 J3789-11 U4752-7 U4769-6 U4769-7 |
Synonyms: CFC3 Protein, Human; MAPKK1 Protein, Human; MEK1 Protein, Human; MEL Protein, Human; MKK1 Protein, Human; PRKMK1 Protein, Human
Image / Experimental Information
| Image 1 Description | Determination of ATP Km for recombinant protein kinases MEK1 (Cat. No.: M02-10G) using ADP-GloTM Assay. With saturating substrate concentration, the relative luminescence values were plotted against various ATP concentrations. The Michaelis-Menten equation was then used to generate the Km values. (Validation Experiment) |
| Image 1 Alt Text | Human MEK1 Protein 46487 |
| Image 1 URL | Source image |
| Image 2 Description | Determination of ATP Km for recombinant protein kinases MEK1 (Cat. No.: M02-10G) using ADP-GloTM Assay. With saturating substrate concentration, the relative luminescence values were plotted against various ATP concentrations. The Michaelis-Menten equation was then used to generate the Km values. (Validation Experiment) |
| Image 2 Alt Text | Human MEK1 Protein 47283 |
| Image 2 URL | Source image |
| Image 3 Description | Determination of ATP Km for recombinant protein kinases MEK1 (Cat. No.: M02-10G) using ADP-GloTM Assay. With saturating substrate concentration, the relative luminescence values were plotted against various ATP concentrations. The Michaelis-Menten equation was then used to generate the Km values. (Validation Experiment) |
| Image 3 Alt Text | Human MEK1 Protein 47284 |
| Image 3 URL | Source image |
Background Information
| Full Name | mitogen-activated protein kinase kinase 1 |
| Description | MEK1, also known as MAP2K1 and MKK1, is a member of the dual-specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. MEK1 is widely expressed, with extremely low levels in the brain. It lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon a wide variety of extra- and intracellular signals. As an essential component of the MAP kinase signal transduction pathway, MEK1 is involved in many cellular processes such as proliferation, differentiation, transcription regulation, and development. Binding extracellular ligands such as growth factors, cytokines, and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1 and MEK2. MEK1 has been shown to export PPARG from the nucleus. The MAPK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis. MKK1 catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Defects in MEK1 can cause Cardiofaciocutaneous Syndrome. |
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| References |
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title: ERK1/2 interaction with DHPS regulates eIF5A deoxyhypusination independently of ERK kinase activity.
authors: Andrew E Becker; Paweł Kochanowski; ...; Jong-In Park
journal: Cell Reports
date: 2024-10-09
pmid: 39392755
cited_by_count: 1
techniques: Clone Assay; Construct; Expressing; Generated; Mutagenesis; Plasmid Preparation; Synthesized
bioz_score: 93
title: Domperidone inhibits cell proliferation via targeting MEK and CDK4 in esophageal squamous cell carcinoma.
authors: Qiang Yuan; Yunshu Shi; ...; Kangdong Liu
journal: Cancer cell international
date: 2024-03-25
pmid: 38528618
doi: 10.1186/s12935-024-03291-8
cited_by_count: 2
techniques: Recombinant
bioz_score: 92
title: Modulation of the proteostasis network promotes tumor resistance to oncogenic KRAS inhibitors.
authors: Xiangdong Lv; Xuan Lu; ...; Xi Chen
journal: Science (New York, N.Y.)
date: 2023-09-11
pmid: 37676964
doi: 10.1126/science.abn4180
cited_by_count: 65
techniques: Autoradiography; In Vitro; Incubation; Kinase Assay; Labeling; Purification; Recombinant
bioz_score: 91
title: Erianin suppresses constitutive activation of MAPK signaling pathway by inhibition of CRAF and MEK1/2
authors: Penglei Wang; Xuechao Jia; ...; Kangdong Liu
journal: Signal transduction and targeted therapy
date: 2023-03-06
pmid: 36872366
doi: 10.1038/s41392-023-01329-3
pmcid: 9986241
cited_by_count: 35
techniques: Activation Assay; Activity Assay; Binding Assay; Concentration Assay; Control; Detection Assay; Expressing; Growth Assay; Immunofluorescence; Immunohistochemistry; Immunoprecipitation; In Vitro; In Vivo; Incubation; Inhibition; Injection; Isothermal Titration Calorimetry; Kinase Assay; Knockdown; Labeling; MTT Assay; Mutagenesis; Over Expression; Pull Down Assay; Recombinant; Rescue Assay; Software; Thermal Shift Assay; Titration; Transfection; Western Blot; shRNA
bioz_score: 92
title: Azelnidipine inhibits esophageal squamous cell carcinoma proliferation in vivo and in vitro by targeting MEK1/2
authors: Lili Zhao; Yuhan Zhang; ...; Kangdong Liu
journal: Molecular therapy oncolytics
date: 2022-09-26
pmid: 36284716
doi: 10.1016/j.omto.2022.09.007
pmcid: 9574346
cited_by_count: 4
techniques: Activity Assay; In Vitro; Incubation; Kinase Assay
bioz_score: 92
title: Perphenazine exerts antitumor effects on HUT78 cells through Akt dephosphorylation by protein phosphatase 2A
authors: Shunya Tsuji; Naoki Kohyanagi; ...; Koichi Sato
journal: Oncology Letters
date: 2021-02-01
pmid: 33376545
doi: 10.3892/ol.2020.12374
pmcid: 7751355
cited_by_count: 6
techniques: Activity Assay; De-Phosphorylation Assay; In Vitro; Incubation; Kinase Assay; Recombinant; Western Blot
bioz_score: 92
title: Acetylshikonin suppressed growth of colorectal tumour tissue and cells by inhibiting the intracellular kinase, T‐lymphokine‐activated killer cell‐originated protein kinase.
authors: Ran Zhao; Bu Young Choi; ...; Mee‐Hyun Lee
journal: British Journal of Pharmacology
date: 2020-04-10
pmid: 31985814
doi: 10.1111/bph.14981
pmcid: 7174886
cited_by_count: 21
techniques: Activity Assay; Binding Assay; Immunohistochemical staining; In Vitro; Inhibition; Kinase Assay; Recombinant
bioz_score: 92
title: Stem Cell Factor-Inducible MITF-M Expression in Therapeutics for Acquired Skin Hyperpigmentation
authors: Cheong-Yong Yun; Eunmiri Roh; ...; Youngsoo Kim
journal: Theranostics
date: 2020-01-01
pmid: 31903124
doi: 10.7150/thno.39066
pmcid: 6929618
cited_by_count: 9
techniques: Enzyme-linked Immunosorbent Assay; In Vitro; Kinase Assay; Radioactivity
bioz_score: 92
title: LIMD1 phosphorylation in mitosis is required for mitotic progression and its tumor-suppressing activity.
authors: Jiuli Zhou; Lin Zhang; ...; Jixin Dong
journal: The Febs Journal
date: 2019-12-13
pmid: 30600590
doi: 10.1111/febs.14743
cited_by_count: 12
bioz_score: 92
title: Purpurogallin is a novel mitogen-activated protein kinase kinase 1/2 inhibitor that suppresses esophageal squamous cell carcinoma growth in vitro and in vivo.
authors: Xiaomeng Xie; Xueyin Zu; ...; Dong Joon Kim
journal: Molecular carcinogenesis
date: 2019-11-07
pmid: 31100197
doi: 10.1002/mc.23007
cited_by_count: 16
bioz_score: 92
title: Discovery of carbazole derivatives as novel allosteric MEK inhibitors by pharmacophore modeling and virtual screening
authors: Dandan Xi; Yan Niu; ...; Ping Xu
journal: European Journal of Medicinal Chemistry
date: 2019-09-01
doi: 10.1016/j.ejmech.2019.06.027
cited_by_count: 14
techniques: Binding Assay; Fluorescence; HTRF Assay
bioz_score: 92
title: Dietary Flavone Tectochrysin Exerts Anti-Inflammatory Action by Directly Inhibiting MEK1/2 in LPS-Primed Macrophages.
authors: Rui Hou; Yixin Han; ...; Yun Qi
journal: Molecular nutrition & food research
date: 2018-12-27
pmid: 28980448
doi: 10.1002/mnfr.201700288
cited_by_count: 25
techniques: Kinase Assay; Recombinant
bioz_score: 92
title: Drug-dependent functionalization of wild-type and mutant p53 in cisplatin-resistant human ovarian tum3or cells
authors: Michelle Bhatt; Cristina Ivan; ...; Zahid H. Siddik
journal: Oncotarget
date: 2017-02-14
pmid: 28038466
doi: 10.18632/oncotarget.14228
pmcid: 5355233
cited_by_count: 16
techniques: Immunoprecipitation; Positive Control; Recombinant
bioz_score: 92
title: Structural and Functional Study of RI alpha -- nucleated PKA Signaling Complexes
authors: Bruystens, Jessica Gundi Helga
date: 2014-01-01
cited_by_count: 0
techniques: Cell Culture; Incubation; Mass Spectrometry; Transfection
bioz_score: 91
title: CDK1 Phosphorylation of YAP Promotes Mitotic Defects and Cell Motility and Is Essential for Neoplastic Transformation
authors: Shuping Yang; Lin Zhang; ...; Jixin Dong
journal: Cancer Research
date: 2013-10-07
doi: 10.1158/0008-5472.can-13-2049
cited_by_count: 96
bioz_score: 92
title: Phospho-regulation of KIBRA by CDK1 and CDC14 phosphatase controls cell-cycle progression
authors: Ming Ji; Shuping Yang; ...; Jixin Dong
journal: Biochemical Journal
date: 2012-09-12
doi: 10.1042/bj20120751
cited_by_count: 0
techniques: In Vitro; Kinase Assay
bioz_score: 92
MDSD
