WB (FAQ Protocol), ELISA (FAQ Protocol), IP (FAQ Protocol)
Species reactivity
Reacts with: Human
Specificity
Human CER1
Immunogen
Recombinant Human CER1 / Cerberus 1 Protein (Catalog#10820-H08B)
Preparation
Produced in rabbits immunized with purified, recombinant Human CER1 / Cerberus 1 (rh CER1 / Cerberus 1; Catalog#10820-H08B; AAH69371.1; Met1-Ala267). CER1 / Cerberus 1 specific IgG was purified by Human CER1 / Cerberus 1 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
Synonyms: Anti-DAND4 Antibody
Image / Experimental Information
Image 1 Description
CER1 was immunoprecipitated using: Lane A:0.5 mg Jurkat Whole Cell Lysate 1 µL anti-CER1 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose. Primary antibody: Anti-CER1 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 30 kDa Observed band size: 34 kDa
Image 1 Alt Text
Human CER1 / Cerberus 1 Immunoprecipitation(IP) 14704
CER1 was immunoprecipitated using: Lane A:0.5 mg Jurkat Whole Cell Lysate 1 µL anti-CER1 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose. Primary antibody: Anti-CER1 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 30 kDa Observed band size: 34 kDa