| Catalog No. | Size | Price (USD) |
| M03-10G-10 | 10 µg | $515.43 |
| M03-10G-50 | 50 µg | $1,729.93 |
| M03-10G-500 | 500 µg | $3,907.16 |
General Information
| Formulation | Supplied as sterile 50 mM Tris-HCl, pH 7.5, 50-300mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25-1.0mM DTT, 0-0.1mM PMSF, 10-25% glycerol Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| Shipping | Enzymes are highly recommended to be shipped at frozen temperature with dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products. |
| Stability & Storage | Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. |
| Purity | For specific information on a given lot, see related technical data sheet. |
| Activity | For specific information on a given lot, see related technical data sheet. |
| Protein Construction | Recombinant full length human MEK2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. |
| Expressed Host | Baculovirus-Insect Cells |
| Species | Human |
| Molecule Mass | ~71 kDa |
| Technical Data Sheet by Lot # | Q2450-7 |
Synonyms: CFC4 Protein, Human; MAPKK2 Protein, Human; MEK2 Protein, Human; MKK2 Protein, Human; PRKMK2 Protein, Human
Image / Experimental Information
| Image 1 Description | Determination of ATP Km for recombinant protein kinases MEK2 (Cat. No.: M03-10G) using ADP-GloTM Assay. With saturating substrate concentration, the relative luminescence values were plotted against various ATP concentrations. The Michaelis-Menten equation was then used to generate the Km values. (Validation Experiment) |
| Image 1 Alt Text | Human MEK2 Protein 46488 |
| Image 1 URL | Source image |
| Image 2 Description | Determination of ATP Km for recombinant protein kinases MEK2 (Cat. No.: M03-10G) using ADP-GloTM Assay. With saturating substrate concentration, the relative luminescence values were plotted against various ATP concentrations. The Michaelis-Menten equation was then used to generate the Km values. (Validation Experiment) |
| Image 2 Alt Text | Human MEK2 Protein 47285 |
| Image 2 URL | Source image |
| Image 3 Description | Determination of ATP Km for recombinant protein kinases MEK2 (Cat. No.: M03-10G) using ADP-GloTM Assay. With saturating substrate concentration, the relative luminescence values were plotted against various ATP concentrations. The Michaelis-Menten equation was then used to generate the Km values. (Validation Experiment) |
| Image 3 Alt Text | Human MEK2 Protein 47286 |
| Image 3 URL | Source image |
Background Information
| Full Name | mitogen-activated protein kinase kinase 2 |
| Description | Dual specificity mitogen-activated protein kinase kinase 2, also known as MAP kinase kinase 2, MAPKK2, ERK activator kinase 2, MAPK / ERK kinase 2, MEK2 and MAP2K2, is a member of the protein kinase superfamily, STE Ser/Thr protein kinase family and MAP kinase kinase subfamily. MAP2K2 / MEK2 contains one protein kinase domain. MEK1 and MEK2 (also known as MAP2K1 and MAP2K2, respectively) are evolutionarily conserved, dual-specificity kinases that mediate Erk1 and Erk2 activation during adhesion and growth factor signaling. MAP2K1 / MEK1 is a crucial modulator of Mek and Erk signaling and have potential implications for the role of MEK1 and MEK2 in tumorigenesis. MAP2K2 / MEK2 catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. It also activates the ERK1 and ERK2 MAP kinases. Defects in MAP2K2 are a cause of Cardiofaciocutaneous Syndrome (CFC Syndrome) which is characterized by a distinctive facial appearance, heart defects, and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects, and hypertrophic cardiomyopathy. |
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| References |
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title: Domperidone inhibits cell proliferation via targeting MEK and CDK4 in esophageal squamous cell carcinoma
authors: Qiang Yuan; Yunshu Shi; ...; Kangdong Liu
journal: Cancer cell international
date: 2024-03-25
pmid: 38528618
pmcid: 10964662
cited_by_count: 2
techniques: Activity Assay; Binding Assay; Concentration Assay; Expressing; In Vitro; Incubation; Kinase Assay; Knock-Out; Knockdown; MTT Assay; Mutagenesis; Pull Down Assay; Recombinant; Transfection; Western Blot
bioz_score: 92
title: Erianin suppresses constitutive activation of MAPK signaling pathway by inhibition of CRAF and MEK1/2
authors: Penglei Wang; Xuechao Jia; ...; Kangdong Liu
journal: Signal transduction and targeted therapy
date: 2023-03-06
pmid: 36872366
doi: 10.1038/s41392-023-01329-3
pmcid: 9986241
cited_by_count: 35
techniques: Activity Assay; Binding Assay; Concentration Assay; Control; Detection Assay; Expressing; Growth Assay; Immunoprecipitation; Incubation; Isothermal Titration Calorimetry; Kinase Assay; Knockdown; Labeling; MTT Assay; Mutagenesis; Over Expression; Pull Down Assay; Recombinant; Rescue Assay; Software; Thermal Shift Assay; Titration; Transfection; Western Blot; shRNA
bioz_score: 92
title: The dynamic mechanism of 4E-BP1 recognition and phosphorylation by mTORC1.
authors: Raphael Böhm; Stefan Imseng; ...; Sebastian Hiller
journal: Molecular cell
date: 2021-06-22
pmid: 33852892
doi: 10.1016/j.molcel.2021.03.031
cited_by_count: 88
techniques: Mutagenesis; Plasmid Preparation; Recombinant
bioz_score: 92
title: Dietary Flavone Tectochrysin Exerts Anti-Inflammatory Action by Directly Inhibiting MEK1/2 in LPS-Primed Macrophages.
authors: Rui Hou; Yixin Han; ...; Yun Qi
journal: Molecular nutrition & food research
date: 2018-12-27
pmid: 28980448
doi: 10.1002/mnfr.201700288
cited_by_count: 25
techniques: Kinase Assay; Recombinant
bioz_score: 92
title: An ERK/Cdk5 axis controls the diabetogenic actions of PPARγ.
authors: Alexander S Banks; Fiona E McAllister; ...; Bruce M Spiegelman
journal: Nature
date: 2015-02-02
pmid: 25409143
doi: 10.1038/nature13887
cited_by_count: 173
techniques: Incubation; Purification; Recombinant
bioz_score: 92
title: CInQ-03, a novel allosteric MEK inhibitor, suppresses cancer growth in vitro and in vivo
authors: Dong Joon Kim; Mee-Hyun Lee; ...; Zigang Dong
journal: Carcinogenesis
date: 2013-05-01
pmid: 23354306
doi: 10.1093/carcin/bgt015
pmcid: 3643416
cited_by_count: 5
techniques: Recombinant
bioz_score: 92
title: Direct Targeting of MEK1/2 and RSK2 by Silybin Induces Cell-Cycle Arrest and Inhibits Melanoma Cell Growth
authors: Mee-Hyun Lee; Zunnan Huang; ...; Zigang Dong
journal: Cancer Prevention Research
date: 2013-02-27
doi: 10.1158/1940-6207.capr-12-0425
cited_by_count: 49
techniques: Activity Assay; Ex Vivo; Expressing; In Vitro; Incubation; Kinase Assay; Pull Down Assay; Recombinant; Software; Western Blot
bioz_score: 92
MDSD
