Supplied as sterile 50 mM Tris-HCl, pH 7.5, 50-300mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25-1.0mM DTT, 0-0.1mM PMSF, 10-25% glycerol Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Shipping
Enzymes are highly recommended to be shipped at frozen temperature with dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Stability & Storage
Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity
For specific information on a given lot, see related technical data sheet.
Activity
For specific information on a given lot, see related technical data sheet.
Protein Construction
Recombinant full length human NEK6 was expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag.
Expressed Host
Baculovirus-Insect Cells
Species
Human
Molecule Mass
~62 kDa
Technical Data Sheet by Lot #
B2117-8 P1737-8 Q128-4
Synonyms: SID6-1512 Protein, Human
Image / Experimental Information
Image 1 Description
Determination of ATP Km for recombinant protein kinases NEK6 (Cat. No.: N08-10G) using ADP-GloTM Assay. With saturating substrate concentration, the relative luminescence values were plotted against various ATP concentrations. The Michaelis-Menten equation was then used to generate the Km values. (Validation Experiment)