| Catalog No. | Size | Price (USD) |
| 50446-M08H-1 | 1 mg | $1,907.67 |
| 50446-M08H-100 | 100 µg | $293.26 |
| 50446-M08H-200 | 200 µg | $573.19 |
General Information
| Formulation | Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Shipping | In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
| Stability & Storage | Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Reconstitution | To find reconstitution info, click on 'COA' at the top of the page, enter the lot number and product size for the lot-specific COA. |
| Purity | ≥ 97 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. |
| Activity | 1.Measured by its ability to bind recombinant mouse CD28 in a functional ELISA. 2.Loaded Recombinant Mouse CD28 Protein, hFc Tag (Cat. No. 50103-M02H) on proA Biosensor, can bind Recombinant Mouse B7-1/CD80 Protein, His Tag (Cat. No. 50446-M08H) with an affinity constant of 1.410 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested). |
| Protein Construction | A DNA sequence encoding the mouse CD80 (Q00609-1) extracellular domain (Met 1-Lys 245) was expressed, with a polyhistidine tag at the C-terminus. |
| Accession# | Q00609-1 |
| Expressed Host | HEK293 Cells |
| Species | Mouse |
| Predicted N Terminal | Val 38 |
| Molecule Mass | The recombinant mouse CD80 consists of 219 amino acids and has a predicted molecular mass of 25.1 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm CD80 is approximately 45-55 kDa due to glycosylation. |
Synonyms: B7 Protein, Mouse; B71 Protein, Mouse; Cd28l Protein, Mouse; Cd80 Protein, Mouse; Ly-53 Protein, Mouse; Ly53 Protein, Mouse; MIC17 Protein, Mouse; TSA1 Protein, Mouse
Image / Experimental Information
| Image 1 Alt Text | Mouse CD80/B7-1 Protein 11643 |
| Image 1 URL | Source image |
| Image 2 Alt Text | Mouse B7-1 Protein 38911 |
| Image 2 URL | Source image |
| Image 3 Alt Text | Mouse CD28 Protein 43076 |
| Image 3 URL | Source image |
Background Information
| Full Name | CD80 molecule |
| Description | The B-lymphocyte activation antigen B7-1 (referred to as B7), also known as CD80, is a member of cell surface immunoglobulin superfamily and is expressed on the surface of antigen-presenting cells including activated B cells, macrophages and dendritic cells. As costimulatory ligands, B7-1 which exists predominantly as dimer and the related protein B7-2, interact with the costimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells, and thus constitute one of the dominant pathways that regulate T cell activation and tolerance, cytokine production, and the generation of CTL. The B7/CD28/CTLA4 pathway has the ability to both positively and negatively regulate immune responses. CD80 is thus regarded as promising therapeutic targets for autoimmune diseases and various carcinomas. |
| Research Areas |
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| Tags |
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| References |
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title: Microdevice for confinement of T-cells on functionalized bio-interfaces.
authors: Christoph Trenzinger; Caroline Kopittke; ...; Mario Brameshuber
journal: Lab on a Chip
date: 2025-04-30
pmid: 40302492
doi: 10.1039/d5lc00248f
cited_by_count: 0
techniques: Clinical Proteomics; Imaging
bioz_score: 93
title: High‐Affinity Superantigen‐Based Trifunctional Immune Cell Engager Synergizes NK and T Cell Activation for Tumor Suppression
authors: Yao‐An Yu; Wan‐Ju Lien; ...; Kurt Yun Mou
journal: Advanced Science
date: 2024-06-27
pmid: 38937984
pmcid: 11434130
cited_by_count: 2
techniques: Amplification; Binding Assay; Cell Culture; Clone Assay; Flow Cytometry; Fluorescence; Incubation; Mutagenesis; Protein Binding; Recombinant; Selection; Staining
bioz_score: 93
title: Strategies for the Site-Specific Decoration of DNA Origami Nanostructures with Functionally Intact Proteins
authors: Joschka Hellmeier; René Platzer; ...; Eva Sevcsik
journal: ACS Nano
date: 2021-08-31
pmid: 34463486
doi: 10.1021/acsnano.1c05411
pmcid: 8482763
cited_by_count: 22
techniques: Activation Assay; Concentration Assay; Construct; Imaging; Incubation; Isolation; Positive Control
bioz_score: 93
title: DNA origami demonstrate the unique stimulatory power of single pMHCs as T cell antigens
authors: Joschka Hellmeier; Rene Platzer; ...; Eva Sevcsik
journal: Proceedings of the National Academy of Sciences of the United States of America
date: 2021-01-26
pmid: 33468643
doi: 10.1073/pnas.2016857118
pmcid: 7848602
cited_by_count: 65
techniques: Activation Assay; Concentration Assay; Construct; Fluorescence; Imaging; Incubation; Labeling; Microscopy; Negative Control; Positive Control
bioz_score: 93
title: A functional antibody cross-reactive to both human and murine cytotoxic T-lymphocyte-associated protein 4 via binding to an N-glycosylation epitope
authors: Dong Li; Jing Li; ...; Zhuozhi Wang
journal: mAbs
date: 2020-01-01
pmid: 32054416
doi: 10.1080/19420862.2020.1725365
pmcid: 7039627
cited_by_count: 14
techniques: Blocking Assay; Competitive Binding Assay; Enzyme-linked Immunosorbent Assay; Incubation; Negative Control; Spectrophotometry
bioz_score: 93
title: PD-L1:CD80 Cis -Heterodimer Triggers the Co-stimulatory Receptor CD28 While Repressing the Inhibitory PD-1 and CTLA-4 Pathways
authors: Yunlong Zhao; Calvin K. Lee; ...; Enfu Hui
journal: Immunity
date: 2019-12-28
pmid: 31757674
doi: 10.1016/j.immuni.2019.11.003
pmcid: 6935268
cited_by_count: 225
techniques: Cell Culture; Cell Isolation; Centrifugation; Co-Culture Assay; Co-culture Assay; Concentration Assay; Enzyme-linked Immunosorbent Assay; Expressing; Flow Cytometry; Fluorescence; Imaging; Incubation; Isolation; Labeling; Microscopy; Recombinant; Software; Staining
bioz_score: 94
title: PD-L1:CD80 Heterodimer Triggers CD28 While Repressing Both PD-1 and CTLA4 Pathways
authors: Zhao Yunlong; Lin Chia-Hao; ...; Hui Enfu
journal: bioRxiv
date: 2019-04-21
doi: 10.1101/615138
cited_by_count: 0
techniques: Binding Assay; Blocking Assay; Cell Culture; Centrifugation; Co-Culture Assay; Co-culture Assay; Concentration Assay; Conjugation Assay; Expressing; Flow Cytometry; Fluorescence; Generated; Imaging; Immunoprecipitation; Incubation; Isolation; Knock-Out; Labeling; Lysis; Microscopy; Purification; Recombinant; Software; Staining; Western Blot
bioz_score: 93
