| Catalog No. | Size | Price (USD) |
| 40150-MM08-20 | 20 µL | $118.49 |
| 40150-MM08-50 | 50 µL | $222.17 |
| 40150-MM08-100 | 100 µL | $370.28 |
General Information
| Product name | SARS-CoV Spike S1 Subunit Antibody, Mouse MAb |
| Validated applications | WB (FAQ Protocol), ELISA (FAQ Protocol) |
| Application notes | IHC, FCM, IF, IP et al. applications haven't been validated. (Antibody's applications haven't been validated with corresponding virus positive samples. Optimal concentrations/dilutions should be determined by the end user.) |
| Specificity | SARS-CoV Spike S1 Subunit Has cross-reactivity in ELISA and WB with SARS-CoV Spike S1 (Cat# 40150-V08B1) No cross-reactivity in ELISA and WB with SARS-CoV Spike RBD (Cat# 40150-V08B2) SARS-CoV-2 (2019-nCoV) Spike S1 (Cat# 40591-V08H1) SARS-CoV-2 (2019-nCoV) Spike RBD (Cat# 40592-V08B) |
| Immunogen | Recombinant SARS-CoV Spike S1 Subunit Protein (Catalog#40150-V08B1) |
| Preparation | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant SARS-CoV Spike S1 Subunit (Catalog#40150-V08B1; AAX16192.1; Met1-Arg667). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Source | Monoclonal Mouse IgG2a Clone #08 |
| Purification | Protein A |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Image / Experimental Information
| Image 1 Description | Anti-SARS-COV S1 mouse monoclonal antibody at 1:1000 dilution. Lane A: SARS-CoV Spike S1 (Cat# 40150-V08B1) (30ng) Lane B: SARS-CoV Spike S1 (Cat# 40150-V08B1) (5ng) Secondary Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. |
| Image 1 Alt Text | SARS Coronavirus spike Western blot (WB) 30043 |
| Image 1 URL | Source image |
Background Information
| Description | The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2 (angiotensin-converting enzyme 2), DPP4 (dipeptidyl peptidase-4), APN (aminopeptidase N), CEACAM (carcinoembryonic antigen-related cell adhesion molecule), Sia (sialic acid), O-ac Sia (O-acetylated sialic acid). The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate. |
| References |
|
title: Coronavirus vaccine
authors: Ugur Sahin; Alptekin Güler; ...; Özlem Türeci
date: 2024-03-12
cited_by_count: 0
techniques: Binding Assay; Flow Cytometry; Incubation; Membrane; Microscopy; Recombinant; Software; Staining
bioz_score: 92
title: Mapping immunological and host receptor binding determinants of SARS-CoV spike protein utilizing the Qubevirus platform.
authors: Carrie Sanders; Aristide Dzelamonyuy; ...; Alain Bopda Waffo
journal: The Journal of Biological Chemistry
date: 2023-12-25
pmid: 37977224
cited_by_count: 2
techniques: Bacteria; Construct; Control; Plasmid Preparation; Recombinant; Sequencing
bioz_score: 91
title: Determinants and Mechanisms of the Low Fusogenicity and High Dependence on Endosomal Entry of Omicron Subvariants
authors: Panke Qu; John P. Evans; ...; John T. Patton
journal: mBio
date: 2023-01-10
pmid: 36625591
doi: 10.1128/mbio.03176-22
pmcid: 9972997
cited_by_count: 25
techniques: Activity Assay; Binding Assay; Clarification Assay; Construct; Expressing; Fluorescence; Immunoprecipitation; Infection; Luciferase; Protease Inhibitor; Purification; SDS Page; Staining; Transfection; Western Blot
bioz_score: 92
title: Evasion of neutralizing antibody responses by the SARS-CoV-2 BA.2.75 variant.
authors: Panke Qu; John P Evans; ...; Shan-Lu Liu
journal: Cell host & microbe
date: 2022-11-14
pmid: 36240764
doi: 10.1016/j.chom.2022.09.015
cited_by_count: 90
techniques: Lysis; Protease Inhibitor; Software; Western Blot
bioz_score: 92
title: Determinants and Mechanisms of the Low Fusogenicity and Endosomal Entry of Omicron Subvariants
authors: Qu Panke; Evans John P.; ...; Liu Shan-Lu
journal: bioRxiv
date: 2022-10-17
cited_by_count: 0
techniques: Clarification Assay; Construct; Control; Expressing; Fluorescence; Immunoprecipitation; Protease Inhibitor; Purification; SDS Page; Staining; Transfection; Western Blot
bioz_score: 92
title: Quantification of SARS-CoV-2 neutralizing antibody by wild-type plaque reduction neutralization, microneutralization and pseudotyped virus neutralization assays.
authors: Kevin R Bewley; Naomi S Coombes; ...; Sue Charlton
journal: Nature Protocols
date: 2021-06-14
pmid: 33893470
cited_by_count: 193
techniques: Chromatin Immunoprecipitation; Flow Cytometry; Magnetic Resonance Imaging; Sequencing; Staining
bioz_score: 92
-40150-MM08-HB14AP0202-WB.png){kind=link}